We narrowed to 13,394 results for: 109

1. ExRai-PKNAR

   Plasmid

   #226631

   Purpose

   Excitation-ratiometric biosensor for monitoring Protein Kinase Novel activity in living cells

   Depositor

   Ju Chen

   Article

   Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

   Insert

   ExRai-PKNAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

   Plasmid

   #137993

   Purpose

   Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PM-ExRai-PKNAR

   Plasmid

   #226633

   Purpose

   Plasma membrane-targeted ExRai-PKNAR

   Depositor

   Ju Chen

   Article

   Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

   Insert

   PM-ExRai-PKNAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Plasma membrane targeting N-terminal 11 amino aci…

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PM-ExRai-PKNAR(T/A)

   Plasmid

   #226634

   Purpose

   Negative-control mutant for PM-ExRai-PKNAR biosensor

   Depositor

   Ju Chen

   Article

   Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

   Insert

   PM-ExRai-PKNAR(T/A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Phosphorylatable Thr of PM-ExRai-PKNAR mutated to…

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET23a-mUbc9

   Plasmid

   #53137

   Purpose

   E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Ubc9 (Ube2i Mouse)
   

   Use

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   protein sequence is identical to human

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

   Plasmid

   #137994

   Purpose

   Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLJM60-Tia1

   Plasmid

   #38243

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Tia1 (Tia1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA.PKD.Y463E

   Plasmid

   #10812

   Depositor

   Alex Toker

   Article

   Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

   Insert

   PKD (PRKD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y463E--active; P467L and L520P

   Promoter

   CMV

   Available since

   Oct. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pLJM60-Tiar

   Plasmid

   #38244

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Tiar (Tial1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

10. ExRai-PKNAR(T/A)

    Plasmid

    #226632

    Purpose

    Negative-control mutant for ExRai-PKNAR biosensor

    Depositor

    Ju Chen

    Article

    Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

    Insert

    ExRai-PKNAR (T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Phosphorylatable Thr mutated to Ala

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVET-tTR-KRAB

    Plasmid

    #11644

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hEF-1alpha, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVPRT-tTR-KRAB

    Plasmid

    #11648

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hPrion, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX3X-RanBP2dFG

    Plasmid

    #53120

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2553-2838)

    Promoter

    tac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM60-Cnbp

    Plasmid

    #38246

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Cnbp (Cnbp Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pIS1-Vim5UTR-renilla

    Plasmid

    #38237

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Vim 5'UTR / Renilla (Vim Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Vim 5' UTR fused to renilla ORF

    Promoter

    Endogenous Vim

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pLJM60-eIF4E

    Plasmid

    #38239

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    eIF4E (Eif4e Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH1-SaSrtA-Q

    Plasmid

    #64980

    Purpose

    Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E108Q

    Promoter

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJA59

    Plasmid

    #59932

    Purpose

    gRNA for cleavage at unc-109(n499) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-109(n499) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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