161,601 results

1. pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

   Plasmid

   #216822

   Purpose

   Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

   Insert

   Tornado-Acyclovir-Broccoli#4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSUMO2_Vitiosangium-bGSDM-thrombin

   Plasmid

   #222099

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Vitiosangium-bGSDM-thrombin ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Nostoc-bGSDM-WELQ

   Plasmid

   #222100

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Nostoc-bGSDM-WELQ ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP1894 - pAAV-AiE2582m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #214573

   Purpose

   AiE2582m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Bosiljka Tasic, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH1371

   Plasmid

   #199631

   Purpose

   Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   GPI
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   Pregf-1

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pRDA_833

   Plasmid

   #216086

   Purpose

   Cas9 [Sp] knockout targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-N1-cofilin

   Plasmid

   #186750

   Purpose

   Expresses wildtype mCherry-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBL102-RUBY

   Plasmid

   #210379

   Purpose

   Expresses betalain biosynthesis genes under CaMV 35S promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   35S-RUBY
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF2093

    Plasmid

    #143337

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFP14 (ZFP14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgCrebbp#1/Cre

    Plasmid

    #193209

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgCrebbp#1 (Crebbp Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRPS5A-PTP-3Xflag-16S rRNA Left_G1397C-ABE

    Plasmid

    #189642

    Purpose

    16S rRNA Left_G1397C-ABE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox and TadA 8e
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397N

    Plasmid

    #189644

    Purpose

    16S rRNA Right_G1397N

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBMN iRFP670-OPTN(D474N)

    Plasmid

    #119693

    Purpose

    For mammalian expression of iRFP-tagged UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    D474N

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3- myc NES-mCE(K294A) NLS

    Plasmid

    #82467

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of KRKY from 573-657

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-FKBP-SAC1-HLx2

    Plasmid

    #108133

    Purpose

    Recruitable SAC1 with extended linker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520):[EAAAR]2:SACM1L(521-587)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PWP2_pCSdest

    Plasmid

    #53946

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PWP2 (PWP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pCXLE-hUL

    Plasmid

    #27080

    Purpose

    Integration-free (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    L-MYC (LIN28A, MYCL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCXLE-hSK

    Plasmid

    #27078

    Purpose

    Integration-free (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    SOX2, KLF4 (KLF4, SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV5)

    Viral Prep

    #20298-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    EYFP (Cre-dependent)

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only