166,667 results

1. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV9)

   Viral Prep

   #162379-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   Syn

   Tags

   None

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

2. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

   Plasmid

   #164458

   Purpose

   AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCXLE-hUL

   Plasmid

   #27080

   Purpose

   Integration-free (episomal) expression of human L-MYC and LIN28

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   L-MYC (LIN28A, MYCL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-HOT_tdTomato

   Plasmid

   #138567

   Purpose

   Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   tdTomato
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-G-Flamp1

   Plasmid

   #188567

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-mCherry

   Plasmid

   #36084

   Purpose

   3rd generation lentiviral plasmid expressing mCherry

   Depositor

   Pantelis Tsoulfas

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK381 (AAVS1 CMV-OsTIR1F74G)

   Plasmid

   #140536

   Purpose

   AAVS1 CMV-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV.TBG.PI.Cre.rBG

   Plasmid

   #107787

   Purpose

   AAV vector expressing Cre from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TBG

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1-alpha-V

   Plasmid

   #27290

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha V (ITGAV Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   Available Since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREP-8xARE-GFP-SV40-BFP

    Plasmid

    #134910

    Purpose

    NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Inserts

    8xARE- minimal promoter - GFP
    TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    8xARE - minimal promoter and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Flpo (AAV1)

    Viral Prep

    #55637-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a

    Tags

    None

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

16. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV5)

    Viral Prep

    #162379-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-2xFLAG-SREBP-2

    Plasmid

    #26807

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    L7V, G76S, K395R and I482L

    Promoter

    CMV

    Available Since

    July 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmiRFP670-N1

    Plasmid

    #79987

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CIDAR MoClo Parts Kit

    Plasmid Kit

    #1000000059

    Purpose

    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli

    Depositor

    Douglas Densmore

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only