We narrowed to 4,385 results for: 187

1. pAV115.loxP

   Plasmid

   #63187

   Purpose

   pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer21 Flag-Sesn2 419A/422A/426A

   Plasmid

   #61870

   Purpose

   Dox inducible lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

   Depositor

   Ming Li

   Article

   Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

   Insert

   Sesn2 (Sesn2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   419A/422A/426A

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. KHBD00327

   Plasmid

   #34450

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG18783 (Kr-h1 Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP-C1-IFT57

   Plasmid

   #218728

   Purpose

   Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

   Insert

   IFT57 (IFT57 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N2-IFT20

   Plasmid

   #218719

   Purpose

   Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT20 (IFT20 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218766

   Purpose

   encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-VSMA*
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37V, D167G, D182S, F183M, L186A, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218767

   Purpose

   encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-pAAFRS-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37G, L71V, D182C, F183Y, L186C, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-C1-IFT38

   Plasmid

   #218723

   Purpose

   Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT38 (CLUAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST_A0A061ACN9_CAEEL (pBS0349)

   Plasmid

   #185187

   Purpose

   For the mammalian expression of the C. elegans protein A0A061ACN9_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   A0A061ACN9_CAEEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBS1385 TDP43 RRM-GFP VLIM-S

    Plasmid

    #118792

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBS1384 TDP43 RRM-GFP FYW-L

    Plasmid

    #118793

    Purpose

    To test the effect of sequence on TDP43 RRM-GFP droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All FYW to L in CTD of TDP43 RRM-GFP reporter

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti_Neo_ZFP64_N_3XFLAG

    Plasmid

    #118700

    Purpose

    Express 3xflag tagged ZFP64

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    ZFP64 (ZFP64 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG was fused to the N terminal of ZFP64 cDNA

    Expression

    Mutation

    Promoter

    EF1A

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1 Flag-Sesn2 419A/422A/426A

    Plasmid

    #61872

    Purpose

    Lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    419A/422A/426A

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. VPS13C^mClover3

    Plasmid

    #118760

    Purpose

    VPS13C internally tagged with mClover3 at residue 1914.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13C (VPS13C Human)
    

    Use

    Tags

    mClover3 inserted at position aa 1914

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Use

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB90 - pAGM4723_VirGN54D

    Plasmid

    #123187

    Purpose

    Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Type

    Empty backbone

    Use

    Level 2 acceptor vector for expression in plants

    Tags

    Expression

    Plant

    Mutation

    VirGN54D for increase virulence

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-EGFP-C-IFT88

    Plasmid

    #218733

    Purpose

    Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT88 (IFT88 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-C1-IFT43

    Plasmid

    #218735

    Purpose

    Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    IFT43 (IFT43 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-EGFP-C-IFT81

    Plasmid

    #218732

    Purpose

    Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT81 (IFT81 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge