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XActB-pSP64T

Plasmid

#32994

Depositor

Sergei Sokol

Article

Sokol et al Cell. 1991 Nov 15;67(4):741-52.

Insert

activin B

Available Since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-LC3-RFP-LC3ΔG

Plasmid

#168997

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC24

Plasmid

#231672

Purpose

Expresses human ZDHHC24 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC24 (ZDHHC24 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pME-3x-FLAG-Ubiquitin (JDW 1098)

Plasmid

#224513

Purpose

A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3x-FLAG-Ubiquitin

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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2xmyc-RAB10

Plasmid

#164631

Purpose

Expresses RAB10 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

Ras-related protein Rab-10 (RAB10 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

Plasmid

#168998

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Unspecified

Tags

EGFP and mRFP1

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244101

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP1

Plasmid

#129499

Purpose

Expresses AausFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1b-ST-WPRE

Plasmid

#214967

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1b-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCardinal (JDW 1418)

Plasmid

#242553

Purpose

Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCardinal

Use

Gateway subcloning

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNCST-AausGFP

Plasmid

#129503

Purpose

Expresses AausGFP constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1s-ST-WPRE

Plasmid

#214968

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1s-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMef2c-AHF-DEST (JDW 476)

Plasmid

#242577

Purpose

Gateway destination vector with the murine Mef2c anterior heart field promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLPCX2-MT1-MMP-eGFP

Plasmid

#89819

Purpose

Express in MT1-MMP mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

MT1-MMP (MMP14 Human)

Use

Retroviral

Tags

eGFP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244099

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244072

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AbKRS-chIPYE

Plasmid

#140020

Purpose

Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)

Tags

FLAG

Expression

Mammalian

Mutation

1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

Promoter

EF1

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-HRD1

Plasmid

#231688

Purpose

Expresses human HRD1 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

HRD1 (SYVN1 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC5

Plasmid

#231654

Purpose

Expresses human ZDHHC5 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC5 (ZDHHC5 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-EGFP-3xHA (JDW 1322)

Plasmid

#224494

Purpose

Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Liefact-EGFP-3xHA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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