We narrowed to 11,304 results for: Vars;

1. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1P shSOD1

   Plasmid

   #102975

   Purpose

   Suppression of SOD1

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shSOD1 (SOD1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_CaMKIIa_SERT_EYFP

   Plasmid

   #198737

   Purpose

   Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

   Depositor

   Lin Tian

   Article

   Vargas et al Science. 2023 Feb 17;379(6633):700-706.

   Insert

   Serotonin transporter and yellow fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC_N_GATA4

   Plasmid

   #167758

   Purpose

   Expresses GATA4 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTPRA_deltaD1-SHC

   Plasmid

   #139615

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   PTPRA_deltaD1 (PTPRA Human)
   

   Use

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Mutation

   D1 domain deletion

   Promoter

   CMV

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC3-FGFR1

   Plasmid

   #222611

   Purpose

   Expression of MAC3-tagged FGFR1

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTPH413

   Plasmid

   #185728

   Purpose

   Bacterial PAM profiling of adenine base editor variants

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Inserts

   ecTadA(8e)-neNme2-C
   Nme2Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TetON-dual-SunTag-Hygro

    Plasmid

    #121119

    Purpose

    Endogenous gene activation with the SunTag CRISPR activation system

    Depositor

    Pablo Navarro

    Article

    Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

    Insert

    dCas9-GCN4x10_scFv-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC_N_KLF9

    Plasmid

    #167774

    Purpose

    Expresses KLF9 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    KLF9 (KLF9 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TUBG1

    Plasmid

    #87954

    Purpose

    expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

    Depositor

    Maria Alvarado-Kristensson

    Article

    Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    whole protein, The C-terminal is a sh-gamma-tubul…

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mDlx-SynTagMA_post-2A-mCerulean

    Plasmid

    #130899

    Purpose

    Synaptic Tag for Mapping Activity in GABAergic neurons

    Depositor

    Simon Wiegert

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    mDlx

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PM-FRB-CFP

    Plasmid

    #67517

    Purpose

    FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

    Expression

    Mammalian

    Mutation

    FRB domain only (residues 2019–2114)

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

15. BirA-Myc_N_MYC

    Plasmid

    #167715

    Purpose

    Expresses MYC in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK1as_T2A_Zeo

    Plasmid

    #118596

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_N_IRF8

    Plasmid

    #167763

    Purpose

    Expresses IRF8 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    IRF8 (IRF8 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RET-V5

    Plasmid

    #139627

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX-FHH-MTORKinaseDomain

    Plasmid

    #120708

    Purpose

    Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MTOR Kinase Domain (MTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 1967-2549

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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