We narrowed to 8,983 results for: ef1;
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Plasmid#128587PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a HP1cs-Frb1x PGK Puro
Plasmid#102808PurposeLentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion proteinDepositorInsertHP1cs-Frb1x
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA
Plasmid#37087PurposeExpresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-mCherry-T2A-RPL22-1xV5
Plasmid#170324PurposeExpresses V5 and mCherry tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode
Plasmid#170228PurposeBarcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorUseLentiviralTagsFLAGExpressionMutationFusionPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-hPOT1_IRES-blast
Plasmid#166411Purposeexpress hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationPromoteref-1aAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC446 - pAAV EF1a hPREP(S554A)
Plasmid#59968PurposeAn AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationS554APromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE-IRES-Blast
Plasmid#213115PurposeExpression of untagged MinE in mammalian cellsDepositorInsertMinE (minE )
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast
Plasmid#71217PurposedCas9 fused to human DNMT3B catalytic domainDepositorInsertdSpCas9-DNMT3B catalytic domain
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE
Plasmid#202617PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependentDepositorInsertFRT-JEDI-1P-Kv
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VGAT-WPRE-pA
Plasmid#39320DepositorInsertSlc32a1 (Slc32a1 Mouse)
UseAAV; RTagsExpressionMutationPromoterAvailable sinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAF0286 Ef1a-Cp36_attB-BFP acceptor
Plasmid#193474PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertCp36_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD
Plasmid#117136PurposeEmpty vector encoding BFP-BSDDepositorInsertBSD2A
UseLentiviralTagsExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-SunTag-RPS7-PP7-WPRE
Plasmid#198338PurposePB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loopsDepositorInsertGCN4x24-RPS7-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loops and SunTagExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_r5-HT1.0
Plasmid#208725PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinaseDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin
Plasmid#115519PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertccdB-sgRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyTagsExpressionInsect and MammalianMutationPromoterEF1aAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-hLMX1A-T2A-copGFP
Plasmid#170444PurposeLentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.DepositorInserthuman LMX1A
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0mut
Plasmid#208724PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only