We narrowed to 1,714 results for: plasmid for e coli

1. AAV-hSynI-rtTAV16

   Plasmid

   #102367

   Purpose

   AAV vector to drive TET-ON activator expression from human synapsin I promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

   Insert

   rtTAV16
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   rtTA variant reported in Gene Therapy (2006) 13, …

   Promoter

   human synapsin I promoter

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SPACE-ΔUGI (pRZ1836)

   Plasmid

   #140243

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CGBE1-VRQR (pBM1192)

   Plasmid

   #140254

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_VRQR-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCN34e-ktrA-yfp

   Plasmid

   #227640

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hisSUMO-mZFP57

   Plasmid

   #87224

   Purpose

   Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli

   Depositor

   Gary Stormo

   Article

   Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

   Insert

   ZFP57 (Zfp57 Mouse)
   

   Use

   Tags

   hisSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST14-SAR1

   Plasmid

   #67388

   Purpose

   Expression of native hSar1 in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCN38-kimA-yfp

   Plasmid

   #227635

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   kimA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCN34e-kimA-yfp

   Plasmid

   #227638

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   kimA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCN38-ktrA-yfp

   Plasmid

   #227636

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST14-SARA

    Plasmid

    #67389

    Purpose

    Expression of native hSara in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJGL004

    Plasmid

    #180608

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    10xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCF1148

    Plasmid

    #222330

    Purpose

    Fluorescent reporter plasmid for Veillonella

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Insert

    bs2 gene with Veillonella parvula SKV38 mdh promoter
    

    Use

    E. coli-veillonella shuttle plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Veillonella parvula SKV38 mdh promoter

    Available since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pSiMPlk_N + pSiMPlk_C

    Plasmid

    #134310

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    kanR_gp41-1 N-intein + kanR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSiMPlc_N + pSiMPlc_C

    Plasmid

    #134312

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSiMPla_N + pSiMPla_C

    Plasmid

    #134314

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    ampR_gp41-1 N-intein + ampR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSiMPlh_N + pSiMPlh_C

    Plasmid

    #134316

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P254Q in hygR_gp41-1 N-intein

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIMAY

    Plasmid

    #68939

    Purpose

    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

    Depositor

    Tim Foster

    Article

    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

    Type

    Empty backbone

    Use

    Staphylococcal allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MK1283

    Plasmid

    #71428

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

    Promoter

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge