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Plasmid#200780PurposePflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFPDepositorInsertGtAR2
UseTagsmCherryExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4609
Plasmid#200781PurposePpdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and other neurons expression of GtACR2 RFPDepositorInsertGtACR2
UseTagsmCherryExpressionWormMutationPromoterPpdf-1Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4140
Plasmid#191479PurposePacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4235
Plasmid#191534PurposePsra-11-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVB/others neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPsra-11Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4253
Plasmid#191539PurposePrig-3-LoxP::EBFP::LoxP::Chrimson::wCherry unc-54 3' UTR C.elegans AVA neuron expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPrig-3Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4211
Plasmid#191532PurposePacr-2s-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans A/B MN expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4217
Plasmid#191533PurposePrig-3-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVA neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPrig-3Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SPLINTR BFP v1
Pooled Library#179776PurposeSPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.DepositorExpressionMammalianUseLentiviralAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-I-PhoCl-HCVp
Plasmid#87697PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cellsDepositorInsertI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
UseTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1
Plasmid#187459PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTEC18
Plasmid#30177DepositorInsertMycobacterium Strong Promoter (MSP)
UseMycobacteria expressionTagsEBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hB
Plasmid#60992PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.DepositorInsertEBFP2
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-B
Plasmid#60978PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EBFP2.DepositorInsertEBFP2
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-NLS
Plasmid#184458PurposeNucleus-targeted EBFP2 expression in mammalian cellsDepositorInsertEBFP2-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-CAAX
Plasmid#184459PurposePlasma membrane-targeted EBFP2 expression in mammalian cellsDepositorInsertEBFP2-CAAX
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-LaminB1
Plasmid#184460PurposeNuclear membrane-targeted EBFP2 expression in mammalian cellsDepositorInsertEBFP2-LaminB1
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mB
Plasmid#60985PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.DepositorInsertEBFP2
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔEC1ΔICD-EB
Plasmid#208522PurposeEBFP2-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsEBFP2ExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-NES
Plasmid#184461PurposeCytosolic EBFP2 expression in mammalian cellsDepositorInsertEBFP2-NES
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_008
Plasmid#123789PurposeEncodes EBFP2 as a Type 3b part to be used in the MTK systemDepositorInsertEBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK332
Plasmid#59573PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5DepositorInsertSGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK333
Plasmid#59574PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5DepositorInsertSGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_009
Plasmid#123956PurposeEncodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertT2A::NLS::EBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_017
Plasmid#123830PurposeEncodes a nuclear localized EBFP2 coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertP2a-NLS::EBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Cytbow
Plasmid#158995PurposeMulticolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Mitbow
Plasmid#158994PurposeMulticolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertMitbow
UseTagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Palmbow
Plasmid#158993PurposeMulticolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)DepositorInsertPalmbow
UseTagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Cytbow
Plasmid#158991PurposeMulticolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)DepositorInsertCytbow
UseTagsThe default expressed EBFP2 is fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPLINTR BFP v1 backbone
Plasmid#179779PurposeSPLINTR backbone vectorDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianMutationPromoterCBh and U6Available sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-FAM13A
Plasmid#185550PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting FAM13ADepositorInsertFAM13A gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-DSP
Plasmid#185549PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting DSPDepositorInsertDSP gRNAs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only