Skip to main content
Addgene
Showing: 241 - 280 of 617 results

  5. GFP-CD8a(7A)

    Plasmid
    #162498
    Purpose
    Expresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a with a mutated stem region (CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine and two serine residues are mu…
    Promoter
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162498-plasmid-map-sequence-id-323489

    Enlarge

  6. SBP-GFP-Tac-TC

    Plasmid
    #162506
    Purpose
    Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162506-plasmid-map-sequence-id-323518

    Enlarge

  20. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Promoter
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

    Enlarge

  23. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Article
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107939-plasmid-map-sequence-id-207466

    Enlarge

  39. pTub-Wg

    Plasmid
    #64321
    Purpose
    Expresses Drosophila Wingless protein under the tubulin promotor
    Depositor
    Roel Nusse
    Article
    Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8
    Insert
    Wingless (wg Fly)
    Use
    Drosophila expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-1 tubulin promoter
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 280 of 617 results