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Showing: 241 - 280 of 374 results

  1. pNB682

    Plasmid
    #60143
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    A4V
    Promoter
    MET17pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60143-plasmid-map-sequence-id-96214

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  3. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

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  4. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  5. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  14. pD649-HAsp-CD7-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156547
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD7 (CD7 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156547-plasmid-map-sequence-id-368522

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  19. pDONR223_EBNA1BP2_WT_V5

    Plasmid
    #82932
    Purpose
    Gateway Donor vector containing EBNA1BP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, David Root, Jesse Boehm
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    EBNA1BP2 (EBNA1BP2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82932-plasmid-map-sequence-id-161987

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  20. pRGW40

    Plasmid
    #12227
    Depositor
    Sung-Hou Kim
    Insert
    pRas189 W40
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Tyr40 to Trp40
    Promoter
    Available since
    Sept. 18, 2006
    Availability
    Academic Institutions and Nonprofits only

  21. pJK241

    Plasmid
    #71693
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Tro404 to Phe
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71693-plasmid-map-sequence-id-129669

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  22. pHH0103 FNBP3 WW domain #1

    Plasmid
    #104233
    Purpose
    Bacterial expression of WW domain #1 from FNBP3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    FNBP3 WW domain #1 (PRPF40A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104233-plasmid-map-sequence-id-204803

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  23. pHH0103 FNBP3 WW domain #2

    Plasmid
    #104234
    Purpose
    Bacterial expression of WW domain #2 from FNBP3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    FNBP3 WW domain #2 (PRPF40A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104234-plasmid-map-sequence-id-204379

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  24. pUASp YFP Cdc42 WT

    Plasmid
    #52248
    Purpose
    Drosophila expression of YFP-Cdc42
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pUASp YFP Cdc42 CA

    Plasmid
    #52250
    Purpose
    Drosophila expression of YFP-Cdc42 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  32. pUASp YFP Rac1 WT

    Plasmid
    #52233
    Purpose
    Drosophila expression of YFP-Rac1
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. HSPA9A

    Plasmid
    #42469
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA9A (HSPA9 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pUASp YFP Rac1 DN

    Plasmid
    #52234
    Purpose
    Drosophila expression of YFP-Rac1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 280 of 374 results