We narrowed to 14,602 results for: 110
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Plasmid#110539PurposeThe plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits
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pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549K 3xFlag
Plasmid#110107Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
TagsFlagExpressionMammalianMutationmutated Asparagine 549 to Lysine (N549K)PromoterCMVAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
TagsFlagExpressionMammalianMutationnonePromoterCMVAvailable SinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (1-517, WT, MG-38-11)
Plasmid#110748PurposeTransient transfection of human USP30DepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BHLHE40(S243A/E248A)
Plasmid#110155PurposeExpression of human BHLHE40 (S243A/E248A) in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
TagsHAExpressionMammalianMutationS243A/E248APromoterCMVAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2
Plasmid#110055PurposeCry1 transcription fluorescence reporterDepositorInsertCry1 promoter and Venus (Cry1 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterCry1Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFR D837A
Plasmid#11014PurposeRetroviral construct for expressing human EGFR with D837A mutation in human cellsDepositorAvailable SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCXCR4-LSSmOrange
Plasmid#110197PurposeEncodes for human CXCR4 coding sequence tagged with the LSS-mOrange FPDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-intron2-Venus-NLS-D2
Plasmid#110057PurposePer2 transcription fluorescence reporterDepositorInsertPer2 promoter and Venus (Per2 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterPer2Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCiPS-NIFV
Plasmid#110807PurposeNon-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cellsDepositorInsertPol
ExpressionMammalianMutation2 mutations in the DDE catalyic core motif of the…PromoterCMVAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3RA-PGK-Puro
Plasmid#110846PurposeLentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralMutationD10APromoterTRE3GAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Leo1
Plasmid#11061DepositorInsertLeo1 (LEO1 Human)
ExpressionMammalianAvailable SinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDEST-periHisMBP
Plasmid#11086DepositorTypeEmpty backboneTags6xHis, MBP, and MBP signal peptideExpressionBacterialPromoterTac (lactose/IPTG inducible)Available SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only