We narrowed to 7,871 results for: 199

1. pET24b-BR1.4

   Plasmid

   #199436

   Purpose

   expresses BAmRed1.4 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMRed1.4
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. V5-LOV-Turbo-NLS

   Plasmid

   #199663

   Purpose

   expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NLS and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNMSB84

   Plasmid

   #199309

   Purpose

   MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

   Depositor

   Michael Krieg

   Article

   Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

   Insert

   flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

   Promoter

   flp-18

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX208_CMV::mCherry-myc-VAMP2

   Plasmid

   #199716

   Purpose

   Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-myc-VAMP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hPU.1ΔN165-pET28b

   Plasmid

   #199796

   Purpose

   Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

   Depositor

   Gregory Poon

   Article

   Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

   Insert

   spi1 (SPI1 Human)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-6xHis-GFP(-30)

   Plasmid

   #199167

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(-30)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. VRK1-dTag-puro

   Plasmid

   #199651

   Purpose

   Expresses VRK1 C-term. Tagged with dTAG

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   VRK1 (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   dTAG

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C2-GGA2

    Plasmid

    #199172

    Purpose

    Expresses GFP-GGA2 fusion in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA2 (GGA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Ala 23 Thr substitution; silent substitution in c…

    Promoter

    CMV

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX NPC1-FLAG-TiD

    Plasmid

    #199748

    Purpose

    Lentiviral construct to stably express NPC1 TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HA-CUL1 Y42A/M43A

    Plasmid

    #19940

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 Y42A/M43A (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    CUL1 with a SKP1 binding mutant

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBT9-NF1-GRD

    Plasmid

    #19993

    Depositor

    Fuyuhiko Tamanoi

    Article

    Morcos et al Mol Cell Biol. 1996 May . 16(5):2496-503

    Insert

    Neurofibromatosis type I (NF1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBP-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199201

    Purpose

    Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-myc3-p27

    Plasmid

    #19937

    Depositor

    Yue Xiong

    Article

    Zariwala et al Oncogene. 1998 Nov 26. 17(21):2787-98.

    Insert

    CDKN1B cyclin-dependent kinase inhibitor 1B (CDKN1B Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Use

    Tags

    his tag and sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LOV-Turbo-V5_pRS415

    Plasmid

    #199667

    Purpose

    expresses LOV-Turbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    Promoter

    Gal1,10

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge