We narrowed to 4,889 results for: phy

1. pEGFPC2-gephyrin C4a

   Plasmid

   #199611

   Purpose

   Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C4a casette insertion

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PHY55-mouse U6-sgLMNA

   Plasmid

   #164045

   Purpose

   U6-driven sgRNA targeting LMNA

   Depositor

   Baohui Chen

   Article

   Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

   Insert

   LMNA targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHyg-AID(31-114)

   Plasmid

   #99514

   Purpose

   Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(31-114) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   Expression

   Mutation

   internal inversion of nucleotides 265-312 of the …

   Promoter

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-hPhyBY276H-mCherry-HRasCT

   Plasmid

   #100540

   Purpose

   Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-908 aa), Y276H muta…

   Promoter

   CAG promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-synp-F-synaptophysin-jRGECO1a

   Plasmid

   #102997

   Purpose

   Expresses synaptophysin fused jRGECO1a under the synapsin promoter

   Depositor

   David Cox

   Insert

   Synaptophysin-jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

   Plasmid

   #125696

   Purpose

   Global labeling of inhibitory synapses (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   mRuby2-Gephyrin.FingR-IL2RGTC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

   Plasmid

   #74317

   Purpose

   A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

   Inserts

   synaptophysin
   DsRedExpress
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   5UAS

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-synp-F-synaptophysin-GCaMP6f

   Plasmid

   #102996

   Purpose

   Expresses synaptophysin fused GCaMP6f under the synapsin promoter

   Depositor

   David Cox

   Insert

   Synaptophysin-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid

    #100281

    Purpose

    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa)

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMiniT:GFP-Gephyrin TKIT donor

    Plasmid

    #169433

    Purpose

    Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-Gephyrin [L106/83R]

    Plasmid

    #182100

    Purpose

    Mammalian Expression Plasmid of anti-Gephyrin (Human). Derived from hybridoma L106/83.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Gephyrin (Homo sapiens) recombinant mouse monoclonal antibody (GPHN Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Amphiphysin Type I/Full Length

    Plasmid

    #47390

    Depositor

    Peter McPherson

    Insert

    Amphiphysin Type I (AMPH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+-Y276H PHYB-CAAX

    Plasmid

    #154911

    Purpose

    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

    Depositor

    Clare Buckley, Jonathan Clarke

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid

    #100282

    Purpose

    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-621 aa)

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHybr_r2a>mG2b-srt-his

    Plasmid

    #124804

    Purpose

    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2b
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFCK(1.3)GFP-SynaptophysW

    Plasmid

    #27235

    Depositor

    Pavel Osten

    Article

    Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Synaptophys-GFP

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHybr_r2a>mG2a-srt-his

    Plasmid

    #124803

    Purpose

    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2a
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHybr_r2a>mG1-srt-his

    Plasmid

    #124802

    Purpose

    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG1
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    -

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFPC2-gephyrin S268A/270A

    Plasmid

    #199608

    Purpose

    Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S268A/S270A

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge