We narrowed to 923 results for: tac promoter

1. pH-01

   Plasmid

   #166936

   Purpose

   Reverse Ptac promoter part (H)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   PTAC promoter - Block H- Flanked by BsaI restriction sites.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-01

   Plasmid

   #166924

   Purpose

   Ptac promoter part (B)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   PTAC promoter - Block B- Flanked by BsaI restriction sites.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EC71174

   Plasmid

   #154067

   Purpose

   Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   HS Cre recombinase
   Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
   HYGROMYCIN Resistance Cassette
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Tags

   Expression

   Bacterial and Plant

   Mutation

   The Arabidopsis thaliana U5 small nuclear ribonuc…

   Promoter

   35S promoter, Hordeum vulgare HSP 17 promoter use…

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCXMG

   Plasmid

   #204409

   Purpose

   Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

   Depositor

   David Ackerley

   Article

   Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF073 MBP-Axin-His

   Plasmid

   #154011

   Purpose

   Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX4T-Mst2 SARAH

   Plasmid

   #84298

   Purpose

   Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

   Depositor

   Jonathan Chernoff

   Article

   Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

   Insert

   Mst2 (STK3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   L443W

   Promoter

   tac

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTHSSe_40

   Plasmid

   #109239

   Purpose

   PTac driving TALEsp2

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   TALEsp2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PTac

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTHSSe_39

   Plasmid

   #109238

   Purpose

   PTac driving TALEsp1

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   TALEsp1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PTac

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTHSSe_37

    Plasmid

    #109237

    Purpose

    PTac driving GFP

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTac

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR - CTRL sgRNA

    Plasmid

    #70662

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pES001 MBP-GSK3β-HA-His

    Plasmid

    #154010

    Purpose

    Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA tag, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF019 MBP-β-catenin-His

    Plasmid

    #154070

    Purpose

    Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMG059 MBP-CREB-His

    Plasmid

    #154076

    Purpose

    Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GST-GIV-CT

    Plasmid

    #69863

    Purpose

    Bacterial expression of GST tagged GIV-CT

    Depositor

    Pradipta Ghosh

    Article

    Garcia-Marcos et al J Biol Chem. 2010 Apr 23;285(17):12765-7

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C terminal amino acids 1623-1870 of human GIV

    Promoter

    TAC

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMG035 MBP-Axin∆BCD-His

    Plasmid

    #154053

    Purpose

    Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF086 MBP-CREB 127-135-His

    Plasmid

    #154075

    Purpose

    Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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