We narrowed to 1,812 results for: plasmid for e coli

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Anti-MBP [N128A/4R]

Plasmid

#206531

Purpose

Mammalian Expression Plasmid of anti-Maltose binding protein (MBP) (E. coli). Derived from hybridoma N128A/4.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Maltose binding protein (MBP) (Escherichia coli) recombinant Mouse monoclonal antibody (malE Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Thioredoxin [N245/44R]

Plasmid

#206546

Purpose

Mammalian Expression Plasmid of anti-Thioredoxin (E. coli). Derived from hybridoma N245/44.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Thioredoxin (Escherichia coli) recombinant Mouse monoclonal antibody (trxA Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pOK12

Plasmid

#49789

Purpose

E. coli cloning vector (KanR, low copy, blue/white selection)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pQE31-6xHis-pHCountdown

Plasmid

#182300

Purpose

E. coli plasmid encoding pH-Countdown with a hexahistidine tag

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH Countdown

Tags

histidine tag

Expression

Bacterial

Promoter

T5

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

Plasmid

#169161

Purpose

To express nsp10-nsp14 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

Plasmid

#169160

Purpose

To express nsp14-nsp10 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET21b(+)-Is-PETase

Plasmid

#112202

Purpose

pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

Depositor

Gregg Beckham, Christopher Johnson

Article

Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

Insert

PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12

Tags

6XHIS

Expression

Bacterial

Promoter

N/A

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-Muc1 mAb

Plasmid

#214954

Purpose

Bacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Muc1 mAb heavy chain (MUC1 )
VNp-anti-Muc1 mAb light chain (MUC1 )

Tags

6xHis and VNp

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIRE4-Bpa

Plasmid

#155342

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

humanized EBpaRS/BstYam

Expression

Mammalian

Promoter

PGK/U6

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA RBS30 sfgfp

Plasmid

#226371

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

copA promoter (pCopA) and RBS30 regulation

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR

Plasmid

#107266

Purpose

mCherry-eDHFR in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

mCherry-eDHFR (dhfr E.coli)

Tags

mCherry

Expression

Mammalian

Promoter

T7

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pXD69m2-AphA

Plasmid

#191247

Purpose

E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA sfgfp

Plasmid

#226374

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with native RBS (ribosome binding site).

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

pCopA

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pUK21

Plasmid

#49788

Purpose

E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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Prham-M.EcoRI-p15A-aadA

Plasmid

#149341

Purpose

expresses M.EcoRI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.EcoRI

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pIA1400

Plasmid

#172519

Purpose

Expression of SARS-COV-2 Nsp12 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCN38-yfp

Plasmid

#227637

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pIA1414

Plasmid

#172402

Purpose

Expression of SARS-COV-2 Nsp9 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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