We narrowed to 10,497 results for: ESP

1. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

   Plasmid

   #229831

   Purpose

   A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

   Plasmid

   #229837

   Purpose

   A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

   Plasmid

   #229838

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexAOp
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

   Plasmid

   #229840

   Purpose

   A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

   Plasmid

   #224491

   Purpose

   Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Golgi-mTagBFP-P2A-H2A-tdiRFP
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

   Plasmid

   #224524

   Purpose

   A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   rox-3xNLS-mCherry-V5-2xSTOP-rox
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-EFS (EF1a core) (JDW 1319)

   Plasmid

   #224474

   Purpose

   Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

   Plasmid

   #224505

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexOP-35SCaMV
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

   Plasmid

   #224506

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-oMDC-LexOP-c-Fos
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

    Plasmid

    #224512

    Purpose

    A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xHA-BirA-T2A-mCherry-Caax
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

    Plasmid

    #224522

    Purpose

    A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOUPc-Rh159-HA

    Plasmid

    #179344

    Purpose

    All-in-on "tet-on" 3G lentiviral vector plasmid encoding rhesus cytomegalovirus Rh159 (NK cell evasion protein) with a C-terminal HA tag fused to its cytoplasmic tail

    Depositor

    Jeremy Kamil

    Article

    Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00

    Insert

    Rh159
    

    Use

    Lentiviral; All in one tet-on lentiviral vector (…

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    tet responsive element 3G

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pinducer 20 DN-KASH

    Plasmid

    #125554

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Mutation

    Truncated form: only the KASH domain of nesprin-…

    Promoter

    tetracycline responsive element

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin nanobody
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

    Plasmid

    #159576

    Purpose

    Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
    

    Tags

    His6

    Expression

    Insect

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TO-RPB1-His WT CRres si2,4R

    Plasmid

    #139404

    Purpose

    Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    pCMV, TRex

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TO-RPB1-His K1268R CRres si2,4R

    Plasmid

    #139405

    Purpose

    Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K1268R

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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