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V406 4HA-CKIe(K38R)

Plasmid

#13725

Depositor

David Virshup

Publication

Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

Insert

casein kinase 1 epsilon (CSNK1E Human)

Tags

4 HA

Expression

Mammalian

Mutation

kinase-inactive due to K38R mutation

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Publication

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Publication

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
mRIP3 GFP wt

Plasmid

#41382

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Publication

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Promoter

pCAGGs

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-PE2-P2A-hMLH1dn

Plasmid

#174827

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-hMLH1dn

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCW57-GFP-P2A-MCS (Neo)

Plasmid

#89181

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Publication

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PGC-1 alpha promoter luciferase delta MEF

Plasmid

#8889

Depositor

Bruce Spiegelman

Publication

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter dMEF (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Site-directed mutagenesis to remove the MEF2 bind…

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-hPDGFRA-GFP

Plasmid

#22919

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Publication

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Publication

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Tags

GFP

Expression

Mammalian

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Publication

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pcDNA3-AKT-PH[R25C]-GFP

Plasmid

#18837

Depositor

Craig Montell

Publication

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

R25C

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCG150

Plasmid

#17247

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

unc-119 rescue fragment in pDESTR4R3

Use

Gateway Cloning

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pINDUCER21-RORA

Plasmid

#51303

Purpose

Expression of RORA after doxycycline administration

Depositor

George Daley

Publication

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

RAR-related orphan receptor A (RORA Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCR-Flag-IKKbeta

Plasmid

#15465

Depositor

Hiroyasu Nakano

Publication

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Tags

Flag

Expression

Mammalian

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
p3xFLAG-CMV10-hAtg13

Plasmid

#22872

Depositor

Noboru Mizushima

Publication

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles