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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pCMV

Plasmid

#8815

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) HIS-tag pET new RBS

Plasmid

#8525

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Contains improved Ribosomal binding site; makes …

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB6 (human) EGFP-fusion

Plasmid

#8535

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains eGFP fused to N-terminal of HOXB6 at AA…

Promoter

CMV

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
p3HAT1Luc1

Plasmid

#236275

Purpose

Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

angiotensin II type 1 receptor (AGTR1 Human)

Use

Tags

3xHA and Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-ColX

Plasmid

#222305

Purpose

Synchronize the trafficking of CollagenX from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)

Use

Tags

Expression

Mammalian

Mutation

signal peptide of COL10A1 removed

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-gp135

Plasmid

#222306

Purpose

Synchronize the trafficking of gp135/podocalyxin from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_sgALDH7A1

Plasmid

#218273

Purpose

CRISPR KO plasmid for ALDH7A1 in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCas_4(I-U)/1_2_VB

Plasmid

#186445

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain swapped with Cas4 domain from G. sulf…

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-Left TALE

Plasmid

#196504

Purpose

Expresses left-side TALE–DddAtox N term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 left TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND2-FusXTBE-Left TALE

Plasmid

#196498

Purpose

Expresses left-side TALE-DddAtox Cterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND2-FusXTBE-Right TALE

Plasmid

#196499

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND2-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND2 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-Left TALE

Plasmid

#196500

Purpose

Expresses left-side TALE-DddAtox Cterm half Cox1-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-Cox1 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cox1-FusXTBE-Right TALE

Plasmid

#196501

Purpose

Expresses Right-side TALE-DddAtox Nterm half Cox1-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-Cox1 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cox1-FusXTBE-Left TALE

Plasmid

#196502

Purpose

Expresses left-side TALE–DddAtox N term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 left TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tl1-FusXTBE-Left TALE

Plasmid

#196506

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 left TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tl1-FusXTBE-RightTALE

Plasmid

#196507

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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