We narrowed to 124,568 results for: CIL

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pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Promoter

Phyper spank

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S3

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Tags

6xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Tags

8xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPSP6+t

Plasmid

#48149

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAB078d9

Plasmid

#79207

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Tags

NLS

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJR2956

Plasmid

#122533

Purpose

Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJAF211

Plasmid

#52407

Purpose

Expresses Flag-tagged mouse Arf6

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf6 (Arf6 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-Purg1-3HA

Plasmid

#39351

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA and kanMX6 module - reverse orientation

Available Since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pAB085d

Plasmid

#79209

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAB088i

Plasmid

#79216

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAB085c

Plasmid

#79208

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ER-BSCaM2

Plasmid

#64855

Purpose

Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-BSCaM2

Tags

CFP, N-terminal 27 amino acids from CYP450, and Y…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJR2955

Plasmid

#122550

Purpose

Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemNeptune2.5

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2-TEV-YFP-Flag

Plasmid

#41004

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGL2

Plasmid

#48742

Purpose

Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

truncated mPer2 promoter/enhancer region (-1128 to +386)

Use

Luciferase

Mutation

truncated promoter region (see pGL6)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pPBpuro-EGFP-eDHFR(69K6)

Plasmid

#178850

Purpose

Mammalian expression of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRVdG-4RFP

Plasmid

#52489

Purpose

Expresses TagRFP-T

Depositor

Sebastian Seung

Article

Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

Insert

TagRFP-T

Use

Rabies virus

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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