We narrowed to 3,238 results for: ER
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Plasmid#206344PurposeAAV plasmid expressing mutant TXNIP in retinal pigment epitheliumDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
Tags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
Tags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneTagsCmyc and HAExpressionYeastAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-HisFlag-USP19ER
Plasmid#155244Purposefor generating adenoviruses expressing the ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseAdenoviralTags6XHis-3XFlagMutationH118R- please see dep. commentsPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER-CAmut
Plasmid#155237Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
Tags6XHis-3XFlagExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
TagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2-R232Q
Plasmid#124064PurposeControl protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATLDepositorInsertcytXlATL2-R232Q
ExpressionBacterialPromotertacAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111922PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μDepositorInsertCeNL(Ca2+)_1.2μ
TagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Ubl4A K48R
Plasmid#61809Purposeexpresses mutated Ubl4A with K48R, with a C-terminal FLAG tagDepositorAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS cSugarCrisp (CT#708)
Plasmid#13956DepositorInsertSugarCrisp (PI15 Chicken)
ExpressionBacterialAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS hSugarCrisp (CT#592)
Plasmid#13966DepositorInsertSugarCrisp (PI15 Human)
ExpressionBacterialAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha D291N mutant
Plasmid#128813PurposeTo make virus expressing Doc2alpha D291 mutantDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPMutationD291NPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta D303N mutant
Plasmid#128815PurposeTo make virus expressing Doc2beta D303N mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPMutationD303NPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha Halotag
Plasmid#128817PurposeTo make virus expressing Doc2alpha HalotagDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsHaloTag-IRES-EGFPPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta Halotag
Plasmid#128818PurposeTo make virus expressing Doc2beta HalotagDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsHaloTag-IRES-EGFPPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
CytERM-mKOkappa
Plasmid#98832PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
TagsmKOkappaExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
CytERM_3xmTurquoise2
Plasmid#112958PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
Tags3x mTurquoise2ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Mfn1-E209A
Plasmid#141155PurposeMammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysisDepositorInsertMFN 1 (MFN1 Human)
TagsAcGFPExpressionMammalianMutationchanged Glutamic acid at position 209 to Alanine …PromoterCMVAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only