316,051 results
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Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV9)
Viral prep#105545-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHITagsEGFPAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorTypeEmpty backboneUseLentiviralAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorTypeEmpty backboneUseLentiviralAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1-FLAG
Plasmid#60360PurposePlasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.DepositorTypeEmpty backboneTagsEGFP and FLAGExpressionMammalianPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1-FLAG
Plasmid#60360PurposePlasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.DepositorTypeEmpty backboneTagsEGFP and FLAGExpressionMammalianPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-RARE-luciferase
Plasmid#13458DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRetinoic Acid Receptor Response Element
UseLuciferaseAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-RARE-luciferase
Plasmid#13458DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRetinoic Acid Receptor Response Element
UseLuciferaseAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe puro HA PIK3CA E545K
Plasmid#12525DepositorInsertPIK3CA E545K (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationE545K. Tumor-derived allele.Available sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBabe puro HA PIK3CA E545K
Plasmid#12525DepositorInsertPIK3CA E545K (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationE545K. Tumor-derived allele.Available sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-tdTomato (AAV PHP.S)
Viral prep#28306-PHP.SPurposeReady-to-use AAV PHP.S particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Plasmid#20297PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorHas serviceAAV1, AAV5, AAV8, AAV9, and AAV RetrogradeInsertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Plasmid#20297PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorHas serviceAAV1, AAV5, AAV8, AAV9, and AAV RetrogradeInsertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUB-GFP
Plasmid#11155PurposeMammalian expression vector for expression of GFP (Ubiquitin C promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin C promoter (UBC Human)
TagsEGFPExpressionMammalianAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUB-GFP
Plasmid#11155PurposeMammalian expression vector for expression of GFP (Ubiquitin C promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin C promoter (UBC Human)
TagsEGFPExpressionMammalianAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by