We narrowed to 4,529 results for: GCA

1. pLKO.1-Tet-Puro-shMMERVK9c_1

   Plasmid

   #185022

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_MMERVK9c
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

   Plasmid

   #168252

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   PBD GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

   Plasmid

   #168253

   Purpose

   "label stable F-actin; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.U6gT28.3.hSyn.H2B.RFP

   Plasmid

   #170370

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

   Plasmid

   #173209

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.U6gT28.13.hSyn.H2B.RFP

   Plasmid

   #170372

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV.U6gT28.3.EFS-NS.H2B-RFP

   Plasmid

   #170364

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bmpr2 gRNA#2

   Plasmid

   #163389

   Purpose

   Cas9-mediated knockout of Bmpr2 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Bmpr2 gRNA#1

   Plasmid

   #163388

   Purpose

   Cas9-mediated knockout of Bmpr2 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tbx21 gRNA#2

    Plasmid

    #170838

    Purpose

    Cas9-mediated knockout of Tbx21 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Tbx21 (Tbx21 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tbx21 gRNA#1

    Plasmid

    #170837

    Purpose

    Cas9-mediated knockout of Tbx21 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Tbx21 (Tbx21 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Bmpr2 gRNA#3

    Plasmid

    #163390

    Purpose

    Cas9-mediated knockout of Bmpr2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-sgCTRL-1

    Plasmid

    #83934

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330_gRNA_IG-DMR-1

    Plasmid

    #159931

    Purpose

    Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse IG-DMR
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. OA-1045D

    Plasmid

    #125006

    Purpose

    Expresses gRNAs targeting hedgehog and wingless

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    wg gRNA (wg Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    u6:3

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px335-sgRNA-EF(Cas9n)-Ddx42-R

    Plasmid

    #122344

    Purpose

    Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ddx42
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px335-sgRNA-EF(Cas9n)-Trim28-R

    Plasmid

    #122339

    Purpose

    Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Trim28
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px335-sgRNA-EF(Cas9n)-Ctcf-L

    Plasmid

    #122319

    Purpose

    Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ctcf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px335-sgRNA-EF(Cas9n)-Etv5-R

    Plasmid

    #122324

    Purpose

    Expresses sgRNA targeting mouse Etv5 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Etv5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. px335-sgRNA-EF(Cas9n)-Peg3-L

    Plasmid

    #122311

    Purpose

    Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Peg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. px335-sgRNA-EF(Cas9n)-Rad21-L

    Plasmid

    #122313

    Purpose

    Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Rad21
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

    Plasmid

    #105031

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTBL436 HEK293site3EQR sgRNA

    Plasmid

    #126445

    Purpose

    To cut the human HEK293 site3 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human HEK293 site3 with NGAG PAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MCM6 E1.2 gRNA

    Plasmid

    #90762

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MCM6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MCM6 (Guide Designation E1.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. DYNLRB1 H11.3 gRNA

    Plasmid

    #90664

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYNLRB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DYNLRB1 (Guide Designation H11.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

    Plasmid

    #121509

    Purpose

    Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah.intron9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. gRNAs[bTub+Tra].1046D

    Plasmid

    #112693

    Purpose

    express two gRNA targeting bTub & Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gh71

    Plasmid

    #106731

    Purpose

    expression of gRNA targeting B3GNT5

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LRG-neo-sgTAF12(mouse)#4.1

    Plasmid

    #105588

    Purpose

    lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting TAF12 HFD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPN018

    Plasmid

    #91664

    Purpose

    Express sgRNA targeting human SCN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SCN2A (SCN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPN279

    Plasmid

    #91654

    Purpose

    Express sgRNA targeting human PTN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    PTN (PTN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CKS2 C8.2 gRNA

    Plasmid

    #90633

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CKS2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CKS2 (Guide Designation C8.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ACTR1B C11.5 gRNA

    Plasmid

    #90524

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ACTR1B

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ACTR1B (Guide Designation C11.5)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RFC4 D6.3 gRNA

    Plasmid

    #90877

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RFC4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RFC4 (Guide Designation D6.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RANGAP1 G6.4 gRNA

    Plasmid

    #90873

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RANGAP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RANGAP1 (Guide Designation G6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. POC1A F12.2 gRNA

    Plasmid

    #90839

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human POC1A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    POC1A (Guide Designation F12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. POC1B C3.4 gRNA

    Plasmid

    #90840

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human POC1B

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    POC1B (Guide Designation C3.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PDCD2L B8.3 gRNA

    Plasmid

    #90833

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PDCD2L

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PDCD2L (Guide Designation B8.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PCNT E2.4 gRNA

    Plasmid

    #90831

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PCNT

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PCNT (Guide Designation E2.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge