We narrowed to 4,733 results for: GCA

Showing: 1441 - 1460 of 4733 results

Results per page:

Sort results by:

pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

Plasmid

#114729

Purpose

3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5, sgRNA M7, sgRNA M9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-blast-cfTuba_1

Plasmid

#26717

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Tuba RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
non-targeting control gRNA (BRDN0001162235)

Plasmid

#80261

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-pegRNA-HEK3-CTTins-px330-scaffold

Plasmid

#180017

Purpose

Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BRD4 gRNA (BRDN0001146786)

Plasmid

#77346

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PIKFYVE gRNA (BRDN0001147303)

Plasmid

#76892

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MET gRNA (BRDN0001147560)

Plasmid

#76061

Purpose

3rd generation lentiviral gRNA plasmid targeting human MET

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MET (MET Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Control-E2F-3'UTR-Mut

Plasmid

#21171

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

E2F 3'UTR Mutant (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

2 miR binding sites mutated. Changed from GCACTTT…

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
PTK2 gRNA (BRDN0001146703)

Plasmid

#75544

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2 (PTK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

Plasmid

#214879

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF2-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK01_Rho_minprox_DsREd

Plasmid

#173489

Purpose

PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Mouse Rhodopsin promoter driving DsRed reporter gene

Expression

Mammalian

Promoter

Mouse Rhodopsin

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD

Plasmid

#169818

Purpose

Expresses C-terminal flag-tagged CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCCC -> AGTC silent mutations at nt527-530 eli…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB09-TRE-beta-globin-miR-124-EF1a-GFP

Plasmid

#117318

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Promoter

Tet-inducible promoter

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2-TGFBR2-m3_3'UTR

Plasmid

#31883

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

TGFBR2 mutant 3'UTR (TGFBR2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Third miR-302b, 372 7-mer seed match mutated from…

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

Plasmid

#224697

Purpose

Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Tags

Gly-Gly-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge