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Flag Intersectin Long (human)

Plasmid

#47393

Depositor

Peter McPherson

Publication

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Publication

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

CUP1

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
Lenti_AID-Dam-lamin B1

Plasmid

#119764

Purpose

Lentiviral plasmid for Mammalian Expression

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam-lamin B1

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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R26P-SA-EGFP-puro

Plasmid

#26890

Depositor

Rudolf Jaenisch

Publication

Blelloch et al Proc Natl Acad Sci U S A. 2004 Sep 28. 1

Insert

EGFP

Use

Mouse Targeting

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

Plasmid

#192664

Purpose

3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al Cell Rep. 2026 Mar 24;45(3):117006. doi:

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, D839A, H840A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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phNFM

Plasmid

#132597

Purpose

Expresses human NFM

Depositor

Anthony Brown

Publication

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFM (NEFM Human)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Publication

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ACE2(-252)-luc

Plasmid

#31111

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Publication

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -252 to +103

Available since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD_GFP11_T7LysH17A

Plasmid

#59591

Purpose

Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

Depositor

Richard Kingston

Publication

Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

Type

Empty backbone

Tags

6xHis-tag and GFP11

Expression

Bacterial

Promoter

pARA

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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EasyFusion mCherry

Plasmid

#112849

Purpose

General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mCherry

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Publication

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Expression

Bacterial

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-HA-TRAIL-R1

Plasmid

#61382

Purpose

Expresses HA tagged TRAIL receptor 1

Depositor

Francis Chan

Publication

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 1

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R1 leader peptide (aa1-23) replaced with TN…

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) prME (JEV) sdm2

Plasmid

#52881

Purpose

expression of Japanese Encephalitis Virus VLPs in mammalian cells

Depositor

Yorgo Modis

Publication

Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

Insert

prME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

sdm2

Promoter

CMV

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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mNeonGreen-KDEL

Plasmid

#226568

Purpose

Encodes mNeonGreen with KDEL ER lumenal target sequence

Depositor

Gia Voeltz

Publication

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

KDEL (ER lumenal target sequence)

Tags

mNeonGreen

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Publication

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v2

Plasmid

#113934

Purpose

Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

Depositor

Yubin Zhou

Publication

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

LOV2 and Stim1 polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI-neo.ARRDC1-mCherry

Plasmid

#38319

Depositor

Quan Lu

Publication

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-EGFP-caax (AAV8)

Viral prep

#190155-AAV8

Purpose

Ready-to-use AAV8 particles produced from AAV_pMBP-EGFP-caax (#190155). In addition to the viral particles, you will also receive purified AAV_pMBP-EGFP-caax plasmid DNA. MBP-driven EGFP expression in oligodendrocytes. These AAV preparations are suitable purity for injection into animals.

Depositor

Brad Zuchero

Publication

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Promoter

MBP

Available since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only
pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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