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Plasmid#211371PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)
Viral Prep#105677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsFusionRed (Cre-dependent)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH (AAV PHP.eB)
Viral Prep#105550-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven expression of Cre. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsNoneAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLychee
Plasmid#219400PurposeConstitutive expression of mLychee in E. coliDepositorInsertmLychee
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase-W159H-S238F
Plasmid#112203PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationW159H, S238FPromoterN/AAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
FSW_jGCaMP8f
Plasmid#197034PurposeExpress GCaMP8f from hSyn1 promoterDepositorInsertGCaMP8f
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B
Plasmid#69281PurposeGolden Gate entry vector; 1st gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B
Plasmid#69282PurposeGolden Gate entry vector; 2nd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1m (AAV9)
Viral Prep#123308-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1m (#123308). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1m plasmid DNA. Synapsin-driven expression of GRAB-NE1m norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Venus (EDCPV)
Plasmid#90085PurposeThis plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianMutationPromoterEFSAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry
Plasmid#92202PurposeFluorescent protein reporter constructDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RP
Plasmid#60497PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV9)
Viral Prep#162377-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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TFORF3343
Plasmid#144819PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRARB (RARB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-E545K
Plasmid#116485PurposeLentiviral expression of PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationE545KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP TMEM41B-GFP
Plasmid#117414PurposeExpresses TMEM41B tagged with GFP in mammalian cellsDepositorInsertTMEM41B (TMEM41B Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only