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MEN2A-MAC

Plasmid

#139634

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Tags

StrepIII-HA-BirA*

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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GRB2-SHN

Plasmid

#139625

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

GRB2 (GRB2 Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pONL-C1_fibrillarin

Plasmid

#65710

Purpose

Expresses ONL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-C1_fibrillarin

Plasmid

#65708

Purpose

Expresses YNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTTCC

Plasmid

#164264

Purpose

Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

Depositor

Gudmundur Hreggvidsson

Article

Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

Inserts

Cas9
gRNA scaffold

Expression

Bacterial

Promoter

Pcel and trc

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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TFEB promoter-luciferase reporter

Plasmid

#66801

Purpose

Reporter for activity of TFEB gene promoter

Depositor

Albert La Spada

Article

Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

Insert

TFEB promoter (Tfeb Mouse)

Use

Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

Plasmid

#239609

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1/7SKhybrid-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239604

Purpose

Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX307 GFP-Kif26b-C

Plasmid

#118483

Purpose

lentiviral expression of GFP-Kif26b-C in mammalian cells

Depositor

Henry Ho

Article

Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

Insert

mouse Kif26b-C (Kif26b Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Encodes GFP fused to amino acid 1735 to 2112 of m…

Promoter

EF1A

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A-L325R

Plasmid

#145373

Purpose

expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

Depositor

Hugues Abriel

Article

Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Expression

Mammalian

Mutation

changed Leucine 325 to Arginine

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified bovine U6-2

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pOD2044-intDEG

Plasmid

#89366

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Pelt-2

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-pcDNA3.1-

Plasmid

#52501

Purpose

expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239610

Purpose

Constitutive active CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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