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pEGFP-N1-AAGAB

Plasmid

#198333

Purpose

Expresses AAGAB-GFP fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1A

Plasmid

#198168

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-TSF-tepsin

Plasmid

#196636

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human tepsin in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-tepsin

Plasmid

#196647

Purpose

Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

tepsin (TEPSIN Human)

Use

Yeast two-hybrid vector

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

silent substitution in codon 199 to eliminate int…

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB201

Plasmid

#169112

Purpose

TESEC expression vector for Alr from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

alr (alr Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB203

Plasmid

#169114

Purpose

TESEC expression vector for mEGFP-Alr fusion construct

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB204

Plasmid

#169115

Purpose

TESEC expression vector for 6xHis-Alr fusion constrcut

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

6xHis-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB205

Plasmid

#169116

Purpose

TESEC expression vector for Asd from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

asd (asd Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB206

Plasmid

#169117

Purpose

TESEC expression vector for CysH from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

cysH (cysH Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB207

Plasmid

#169118

Purpose

TESEC expression vector for DapB from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

dapB (dapB Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB208

Plasmid

#169119

Purpose

TESEC expression vector for TrpD from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

trpD (trpD Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE669

Plasmid

#153235

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE672

Plasmid

#153238

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE473

Plasmid

#153264

Purpose

M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE500

Plasmid

#153273

Purpose

M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE502

Plasmid

#153275

Purpose

M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-vdh

Plasmid

#128134

Purpose

The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

vdh

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-bphD

Plasmid

#128138

Purpose

The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

bphD

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGCS-N2(3xHA)

Plasmid

#85719

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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