We narrowed to 29,288 results for: nes

1. TRE3GV-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222503

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TRE3GV-SpdCas9-Dnmt3A/3L-V2

   Plasmid

   #222505

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRE3GV-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222506

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

   Plasmid

   #222508

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; C706A and R832…

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-RPS14(WT)-Myc

   Plasmid

   #122236

   Purpose

   expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-FLAG-RPS14(89-151)

   Plasmid

   #122299

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 88

   Promoter

   CMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FLAG-RPS14(2-88)

   Plasmid

   #122298

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (partial) (RPS14 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 89 to 151

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLfa1180fa

    Plasmid

    #107615

    Purpose

    Shuttle vector for easy cloning into Minos/3xP3 transgenesis vectors

    Depositor

    Michalis Averof, Ernst Wimmer

    Article

    Horn et al Dev Genes Evol. 2000 Dec;210(12):630-7.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICSL60004

    Plasmid

    #117519

    Purpose

    rbcS-E9 terminator Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GCTT:E9t:CGCT:BsaI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BCJJ163

    Plasmid

    #117512

    Purpose

    AtRPS5a promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pRPS5a:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5a

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LBJJ491

    Plasmid

    #117513

    Purpose

    AtYAO promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pYAO:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtYAO

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LBJJ433

    Plasmid

    #117507

    Purpose

    AtEC1.2 promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pEC1.2:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSPL10p::SPL10-GFP

    Plasmid

    #27406

    Depositor

    David Bartel

    Article

    Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

    Insert

    SPL10 (AT1G27370 Mustard Weed)
    

    Use

    Used to create transgenic lines

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICSL12022

    Plasmid

    #117510

    Purpose

    AtAG promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pAG:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtAG

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SPL11F15R14::pBIB-BAS (gSPL11)

    Plasmid

    #27398

    Depositor

    David Bartel

    Article

    Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

    Insert

    SPL11 (AT1G27360 Mustard Weed)
    

    Use

    Used to create transgenic lines

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSPL10F13R13::pBIB-BAS (gSPL10)

    Plasmid

    #27407

    Depositor

    David Bartel

    Article

    Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

    Insert

    SPL10 (AT1G27370 Mustard Weed)
    

    Use

    Used to create transgenic lines

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICSL12015

    Plasmid

    #117506

    Purpose

    AtUBI10 promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pUBI10:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LBJJ432

    Plasmid

    #117508

    Purpose

    AtEC_enh. promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pEC_enh:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    EC1.2:EC1.1

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge