We narrowed to 11,816 results for: nts
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Plasmid#90412PurposeA FRET sensor that report the electric potential at the inner leaflet of cell plasma membraneDepositorInsertLck10+ Venus+mcherry+ strech of positively charged amino acids
ExpressionMammalianAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KozATG-dL5-2XG4S-mCer3
Plasmid#73207PurposeExpresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)DepositorInsertKozATG-dL5-2XG4S-mCer3
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRCH
Plasmid#136923PurposeExpresses ABEmax-NRCH in mammalian cellsDepositorInsertABEmax-NRCH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAP05
Plasmid#102920PurposeDirect cloning and broad-host-range heterologous expression vectorDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
OeNL/pcDNA3
Plasmid#85202PurposeOrange color variant of bright luminescent proteinDepositorInsertOrange enhanced Nano-lantern
ExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2
Plasmid#80275PurposeGAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
ExpressionInsectAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2303
Plasmid#91068PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRPromoterCmYLCVAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB453
Plasmid#74835PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ERr
Plasmid#61170PurposeFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoterDepositorInsertAtWAK2-mCherry-HDEL
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceApril 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_220
Plasmid#91113PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: noneDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_220d
Plasmid#91114PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: noneDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorInsertAIMTOR A757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1110
Plasmid#91031PurposeModule A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSPDepositorInsertTaCas9
UseCRISPRPromoterZmUbiAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
ePPE
Plasmid#183095PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
ExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50456PurposeDouble floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoterDepositorInsertrM3D-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-eGFP
Plasmid#62518PurposeExpresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.DepositorInserteGFP
UseAAVExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
p6345 MSCV-CMV-Flag-HA-Brd4 1-722
Plasmid#31352DepositorInsertBrd4 1-722 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutation*see commentsAvailable SinceOct. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ETS1_WT
Plasmid#82118PurposeGateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-eYFP
Plasmid#104056PurposeAn AAV genome with tet-inducible expression of the fluorescent protein eYFPDepositorInsertEYFP
UseAAVPromoterTREAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only