We narrowed to 27,003 results for: tat

1. pT2/shCol1a1/Scarlet-Seq1.1

   Plasmid

   #201401

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2/Col1a1/GFP4_Seq 1.1

   Plasmid

   #201400

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDA2ScIfm

   Plasmid

   #193786

   Purpose

   Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTATAA synthetic promoter carrying binding sites…

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKIF1C_P176L-mCherry-FRB

   Plasmid

   #185983

   Purpose

   Mammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   mCherry and FRB

   Expression

   Mammalian

   Mutation

   P176L - mutation causing HSP (SPG58)

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-M13-6His-KIF1C_R169W-GFP

   Plasmid

   #185979

   Purpose

   Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   6xHis and eGFP

   Expression

   Insect

   Mutation

   R169W - mutation causing HSP (SPG58)

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-M13-6His-KIF1C_P176L-GFP

   Plasmid

   #185978

   Purpose

   Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   6xHis and eGFP

   Expression

   Insect

   Mutation

   P176L - mutation causing HSP (SPG58)

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMT Flag-URH49 MUT

   Plasmid

   #110126

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 amino acid mutations (RSFS motif changed to KSL…

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pMT Flag-Hel25E MUT

   Plasmid

   #110122

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KKLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCHKO_HPRT1_exon3_1_As

   Plasmid

   #155057

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-001428(753)-VC

    Plasmid

    #123256

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Use

    Tags

    CD5 leader peptide and VC (Venus residues D155–K2…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; C-terminal Env re…

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF1-ADAM29 (S112F)

    Plasmid

    #31145

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Serine 112 changed to Pheylalnine

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-ADAM7 (S703N)

    Plasmid

    #31141

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM7 (ADAM7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S703N

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

14. BAP1

    Plasmid

    #81024

    Purpose

    Retroviral vector containing human BAP1 and Thy1.1 selection marker

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX313-Firefly luciferase

    Plasmid

    #118017

    Purpose

    Constitutive expression of Firefly luciferase

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX313-Renilla luciferase

    Plasmid

    #118016

    Purpose

    Constitutive expression of Renilla luciferase

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-GTF2IRD1__ALK

    Plasmid

    #116821

    Purpose

    Lentiviral expression of GTF2IRD1__ALK fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    GTF2IRD1__ALK
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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