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We narrowed to 11,383 results for: mpl

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Showing: 2221 - 2250 of 11383 results

  1. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196078-plasmid-map-sequence-id-388989

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  8. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  15. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170818-plasmid-map-sequence-id-334731

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  20. gWizPlum

    Plasmid
    #74475
    Purpose
    Expression of Plum fluorescent protein in mammalian cells
    Depositor
    Loree Heller
    Insert
    mPlum fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV+intron
    Available Since
    April 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  22. pD649-HAsp-SIGLEC9-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156771
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC9 (SIGLEC9 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156771-plasmid-map-sequence-id-393028

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  26. pST50Trc1-DHFRHIS

    Plasmid
    #63949
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63949-plasmid-map-sequence-id-177355

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  29. pST50Trc3-HISNDHFR

    Plasmid
    #63976
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63976-plasmid-map-sequence-id-177382

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  30. pST50Trc2-HISNDHFR

    Plasmid
    #63958
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63958-plasmid-map-sequence-id-177364

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Showing: 2221 - 2250 of 11383 results