We narrowed to 7,871 results for: 199

1. Nme2Cas9_pMCSG7

   Plasmid

   #119925

   Purpose

   Nme2Cas9 bacterial expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXRE5-Z

   Plasmid

   #112509

   Purpose

   Reporter plasmid, responsive to activated Ah receptor/Arnt

   Depositor

   Charles Miller

   Article

   Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

   Insert

   RW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   5 XREs+ minimal Cytochrome C

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mscv2.2-NLRC4

   Plasmid

   #60199

   Purpose

   Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

   Depositor

   Russell Vance

   Article

   Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

   Insert

   NLRC4 (Nlrc4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nme2sgRNA_pLKO

   Plasmid

   #119926

   Purpose

   Nme2Cas9 U6-driven sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   NmeCas9 sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP FRNK

   Plasmid

   #50518

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   truncated (NT2186-3268)

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. human PNP non-tagged

   Plasmid

   #64199

   Purpose

   Expression of native human PNP (non-tagged)

   Depositor

   Vern Schramm

   Article

   Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

   Insert

   Purine Nucleoside Phosphorylase (PNP Human)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-RosaR4 KKR mutations

   Plasmid

   #37199

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Insert

   FokI (fused to RosaR4 ZFN)
   

   Use

   Zfn expression

   Tags

   HA, ROSA26 eZFNs R4, and SV40 NLS

   Expression

   Mammalian

   Mutation

   I538K, E490K, and H537R

   Promoter

   CMV

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K both

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5_tAKARβ

   Plasmid

   #119914

   Purpose

   Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARβ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. epB_UbC_tdMS2cp-tagRFP-Tx2

    Plasmid

    #119908

    Purpose

    ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    HA-tdMS2cp-tagRFP-Tx2-nls
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SHC003BSD-DelGFP

    Plasmid

    #133301

    Purpose

    EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Fluorescent reporter for MOBE1-2

    Plasmid

    #219945

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_tAKARα

    Plasmid

    #119913

    Purpose

    Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEm-CoilE-Actin

    Plasmid

    #181993

    Purpose

    CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    mEm-CoilE-Actin
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-NUDT5

    Plasmid

    #219954

    Purpose

    For gateway cloning of NUDT5

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    NUDT5 (NUDT5 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP FAT

    Plasmid

    #50517

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

    Insert

    protein tyrosine kinase, domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    truncated (NT2630-3268)

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Fluorescent reporter for MOBE3-4

    Plasmid

    #219946

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH47732:PtFCP:ShBle

    Plasmid

    #104893

    Purpose

    Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    ShBle
    

    Use

    Phaeodactylum; golden gate assembly

    Tags

    PtFCP promoter and terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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