We narrowed to 9,540 results for: pcas

1. pCAG-HA-rat Nrxn3beta AS4(-)

   Plasmid

   #58269

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn3beta AS4(-) (Nrxn3 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-mTagBFP2-T2A-iCre

   Plasmid

   #113837

   Purpose

   Monomeric TagBFP2 and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-pac-T2A-FLPo

   Plasmid

   #124835

   Purpose

   Puromycin resistance gene and FLP recombinase

   Depositor

   Mario Capecchi

   Insert

   pac-T2A-FLPo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-RFP-miR-Scrint

   Plasmid

   #19825

   Depositor

   Zuoshang Xu

   Article

   Qiu et al BMC Biotechnol. 2008 . 8():77.

   Insert

   Scrambled miRNA
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pCAX APPs-695-alpha

   Plasmid

   #30147

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein - soluble (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   amino acids 1–612

   Promoter

   Available since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-SARS2-S-D614 (C-flag)

   Plasmid

   #156420

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Tet-on Advanced -BGH-PA

   Plasmid

   #114379

   Purpose

   Express reverse tetracycline inducible rtTA transcriptional activator

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

   Insert

   rtTA and BGH-pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-CRY2-mCherry-MYPT169

   Plasmid

   #178526

   Purpose

   Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry-MYPT169
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-VPR-dSpCas9[StaPL(TI)]

   Plasmid

   #111508

   Purpose

   Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

   Depositor

   Michael Lin

   Article

   Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

   Insert

   VPR-dSpCas9[StaPL(TI)]
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The HCV NS3 protease carries F43L, Q80K, and S122…

   Promoter

   PGK

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-tagRFP-NLS-HA-Bxb1

    Plasmid

    #65625

    Purpose

    Vector for cotranslational expression of tagRFP-Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Bxb1 integrase
    

    Use

    Tags

    HA, SV40 NLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid

    #100540

    Purpose

    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa), Y276H muta…

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Osa-pCAMBIA-1301-STTM319

    Plasmid

    #84201

    Purpose

    Disturb the expression of miR319 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR319
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-Flag-hsDicer (D1709A)

    Plasmid

    #41588

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1709A

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-HIF1 alpha K532R

    Plasmid

    #21102

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-MCP-VPR-2AmScarlet

    Plasmid

    #121964

    Purpose

    Mammalian expression, Transcription regulation module

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cmv d0 dPspCas13b-GGS-Sun

    Plasmid

    #119858

    Purpose

    Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-Sun
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Sox2-IRES-Neo

    Plasmid

    #13462

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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