164,717 results

1. pBA560

   Plasmid

   #186236

   Purpose

   pTet-eLbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV9)

   Viral Prep

   #104492-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Tags

   None

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pPB_mU6_enh-gRNA_Puro-T2A-BFP

   Plasmid

   #235571

   Purpose

   Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   sgRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV enAsCas12f-YHAM-sgRNA_v7

   Plasmid

   #208356

   Purpose

   Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-YHAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F48Y/S188H/V232A/E316M

   Promoter

   CMV

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF1665

   Plasmid

   #141863

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TP73 (TP73 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

   Viral Prep

   #20298-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-N1_hTRIM32

   Plasmid

   #69541

   Purpose

   Expresses human TRIM32 fused to c-terminal EGFP

   Depositor

   Martin Dorf

   Article

   Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

   Insert

   tripartite motif containing 32 (TRIM32 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRE3G-Bi-α-chain

   Plasmid

   #133730

   Purpose

   Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   a-Chain_gePSI
   ZsGreen1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Only one part of the gePSI-system (aa1-141 + stop…

   Promoter

   pTRE3G-Bi

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSelAct-Express

    Plasmid

    #174375

    Purpose

    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible

    Depositor

    Gitta Coaker

    Article

    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

    Type

    Empty backbone

    Use

    Expression vector for targeted integration

    Tags

    Expression

    Mutation

    Promoter

    pCMP1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge