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HA-merlin

Plasmid

#32836

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Merlin (NF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
pBabepuro3-p16Flag

Plasmid

#24934

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p16 (CDKN2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3-KRAS

Plasmid

#13919

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

KRAS 3'UTR (KRAS Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMZ7

Plasmid

#223180

Purpose

pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

Depositor

Paul Blainey

Article

Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

Type

Empty backbone

Use

Chromosomal integration

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK2

Plasmid

#199641

Purpose

Expresses VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-VPS35

Plasmid

#21689

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG wildtype, catalytic dead lipin 1

Plasmid

#32006

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D712E and D714E

Promoter

CMV, SP6

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

Plasmid

#113436

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBLO 64.1

Plasmid

#123120

Purpose

E. coli expression vector for active deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Promoter

NA

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Tags

6xHis-TEV protease

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG wildtype, catalytic active lipin 1

Plasmid

#32010

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Mutation

Mutated amino acids in catalytic triad to alanine…

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

Plasmid

#136514

Purpose

Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

Depositor

Erik Sontheimer

Article

Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

Insert

Codon-optimized AcrIIA5

Use

Lentiviral

Tags

FLAG/NLS

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP6

Plasmid

#178005

Purpose

Expresses N-terminally mEGFP-tagged PARP6

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP6 (PARP6 Human)

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP11

Plasmid

#177998

Purpose

Expresses N-terminally mEGFP-tagged PARP11

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP11 (PARP11 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Danio alpha A-crystallin 1kb promoter/AcGFP

Plasmid

#98096

Purpose

aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha A-crystallin 1 kb fragment (-1028/-1)

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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MIGR1-cyclin E-AA

Plasmid

#47498

Purpose

MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons

Depositor

Alex Minella

Article

Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.

Insert

cyclin E1 (CCNE1 Human)

Use

Retroviral; Mscv-ires-gfp

Expression

Mammalian

Mutation

threonines 62 and 380 to alanine (T62A T380A)

Promoter

LTR

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only