We narrowed to 24,855 results for: Spr

1. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196082

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Aminoglycoside phosphotransferase
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196080

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   Blasticidin S deaminase
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9

   Plasmid

   #42876

   Purpose

   Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   tracr/Cas9
   

   Use

   CRISPR; E.coli

   Expression

   Bacterial

   Available Since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRH030

   Plasmid

   #159633

   Purpose

   CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

   Depositor

   Robert Hertel

   Article

   Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

   Type

   Empty backbone

   Use

   CRISPR; Shuttle vector

   Promoter

   Constitutive tracrRNA

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. phU1-CasRx-non-targeting (pKW82)

   Plasmid

   #229050

   Purpose

   Cloning backbone for CasRx crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. phU1-PspCas13b-non-targeting (pEZ36)

   Plasmid

   #229053

   Purpose

   Cloning backbone for PspCas13b crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLH-sgRNA1-2XMS2

   Plasmid

   #75389

   Purpose

   sgRNA1-2XMS2

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLH-sgRNA1-2XPP7

   Plasmid

   #75390

   Purpose

   sgRNA1-2XPP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVT36b

   Plasmid

   #184437

   Purpose

   CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

   Depositor

   Huimin Zhao

   Article

   Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLH-sgRNA1-2XboxB

    Plasmid

    #75391

    Purpose

    sgRNA1-2XboxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XboxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRGE31

    Plasmid

    #50929

    Purpose

    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-PspCas13b-non-targeting (pJK5)

    Plasmid

    #229052

    Purpose

    Cloning backbone for PspCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmU1-CasRx-non-targeting (pKW83)

    Plasmid

    #229051

    Purpose

    Cloning backbone for CasRx crRNA (Mouse U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-ACME

    Plasmid

    #186717

    Purpose

    ACME dual-deaminase CRISPR base editor

    Depositor

    Xue Gao

    Article

    Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

    Insert

    ACME
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKI1.1

    Plasmid

    #85807

    Purpose

    CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Promoter

    AtRPS5A

    Available Since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMZ288

    Plasmid

    #59897

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMZ289

    Plasmid

    #59898

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMZ381

    Plasmid

    #59899

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEL082

    Plasmid

    #137891

    Purpose

    iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-CasRx-non-targeting (pEZ18)

    Plasmid

    #229049

    Purpose

    Cloning backbone for CasRx crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge