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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,900 results for: pcas

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Showing: 1521 - 1540 of 5900 results

  7. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  17. Myc-gamma1 (in pCAGGS)

    Plasmid
    #118039
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118039-plasmid-map-sequence-id-228636

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Showing: 1521 - 1540 of 5900 results