We narrowed to 2,452 results for: LRE

1. pmCherry-N1-Cofilin-S41E

   Plasmid

   #186751

   Purpose

   Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed serine 41 to glutamic acid

   Promoter

   CMV

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

   Plasmid

   #206093

   Purpose

   Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

   Plasmid

   #206098

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV/B-actin

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgErich3#1/Cre

   Plasmid

   #193211

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgErich3#1 (4922501L14Rik Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgErich3#2/Cre

   Plasmid

   #193212

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgErich3#2 (4922501L14Rik Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

   Plasmid

   #187647

   Purpose

   Encodes gRNA to correct the SFTPC I73T mutation

   Depositor

   Darrell Kotton

   Article

   Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

   Insert

   I73T gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

   Plasmid

   #176144

   Purpose

   EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB-T2A-SIRT6 (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in Gly60 to Ala, and a mutation in the P…

   Promoter

   EF1A

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

   Plasmid

   #176143

   Purpose

   EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB-T2A-SIRT6 (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in Arg65 to Ala, and a mutation in the P…

   Promoter

   EF1A

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

   Plasmid

   #176139

   Purpose

   EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   XRCC1-T2A-PolB (XRCC1, POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP and MYC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid

    #176089

    Purpose

    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…

    Promoter

    CMV

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIGF1_P1 Promoter-FLuc2P

    Plasmid

    #154261

    Purpose

    Destabilized firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 canonical P1 Promoter (IGF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human IGF-1 P1 Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIGF1_P2 Promoter-FLuc2P

    Plasmid

    #154262

    Purpose

    Destabilized firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human IGF-1 P2 Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

    Plasmid

    #159631

    Purpose

    expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

    Depositor

    Andrew Allen, Ross Bathgate

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    Ast (AstA Fly)
    

    Use

    Lentiviral

    Tags

    HA, IRES, mCherry, and neurophysin-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MBP-rPKL S12TAG

    Plasmid

    #107155

    Purpose

    MBP-rPKL construct for production and purification of rat PKL pS12

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 12 to TAG for phosphoserine insertion

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MBP-rPKL S113TAG

    Plasmid

    #107157

    Purpose

    MBP-rPKL S113TAG for production and purification of rat PKL pS113

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 113 changed to TAG for phosphoserine inser…

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-Puro-CLDN18

    Plasmid

    #69466

    Purpose

    Expresses CLDN18

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    CLDN18 (CLDN18 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-Puro-ARHGAP26

    Plasmid

    #69467

    Purpose

    Expresses ARHGAP26

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    ARHGAP26 (ARHGAP26 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJOP-HTT-HR65Q

    Plasmid

    #92249

    Purpose

    HTT donor DNA with 65Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJOP-HTT-HR45Q

    Plasmid

    #92248

    Purpose

    HTT donor DNA with 45Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

    Plasmid

    #223959

    Purpose

    Dual fluorescent reporter for histone H2B and for APC/C activity

    Depositor

    Hee Won Yang

    Article

    Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

    Insert

    H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJOP-HTT-HR81Q

    Plasmid

    #92250

    Purpose

    HTT donor DNA with 81Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJOP-HTT-HR30Q

    Plasmid

    #92247

    Purpose

    HTT donor DNA with 30Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

    Plasmid

    #184556

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLX311-SHOC2-V5

    Plasmid

    #134521

    Purpose

    Lentiviral plasmid expressing SHOC2 with V5 C-term tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    EF1alpha

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223-CDK9

    Plasmid

    #23701

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CDK9 (CDK9 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid

    #184830

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223-CDK5

    Plasmid

    #23699

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid

    #184831

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135766

    Purpose

    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge