We narrowed to 9,242 results for: pho;

1. pGEX Sh3bp2-SH2

   Plasmid

   #46484

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Sh3bp2 (SH3BP2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 450-561

   Promoter

   Tac

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETDuet1-WT-LanCL2-C-Ter-His

   Plasmid

   #154186

   Purpose

   To express LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMyc1ElbLuc,

   Plasmid

   #53243

   Purpose

   reporter plasmid containing 1 myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMyc1ElbLuc (MYC Human)
   

   Use

   Tags

   Expression

   Mutation

   To prepare plasmids with one, two, three, and fou…

   Promoter

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pRetro-XT-PAK2-CFP(puro)-L107F

   Plasmid

   #81180

   Purpose

   Retroviral vector for expression of kinase active PAK2 with a CFP fusion

   Depositor

   Angeliki Malliri

   Article

   Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

   Insert

   p21 (RAC1) activated kinase 2 (PAK2 Human)
   

   Use

   Retroviral

   Tags

   CFP

   Expression

   Mutation

   L107F

   Promoter

   P-tight TRE

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pCIG-Flag-Sox9-IRES-nls-GFP

   Plasmid

   #44281

   Depositor

   Martin Cheung

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

   Insert

   Flag-Sox9 (SOX9 Chicken)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE enhancer and Chicken beta-actin promoter

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCS2-ER-EmEGFP

   Plasmid

   #169000

   Purpose

   Express ER-EmEGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
   

   Use

   Unspecified

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-MSP1D1deltaH4/2

   Plasmid

   #71712

   Purpose

   Membrane scaffold protein (MSP), half of helix 4 truncated

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRRL-VinculinVenus-S1033A

   Plasmid

   #211896

   Purpose

   Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033A (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to alanine (S1033A),…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRRL-VinculinVenus-S1033D

   Plasmid

   #211897

   Purpose

   Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

   Depositor

   Brenton Hoffman

   Article

   Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

   Insert

   VinculinVenus-S1033D (VCL Chicken)
   

   Use

   Lentiviral

   Tags

   VenusA206K

   Expression

   Mutation

   mutated vinculin serine 1033 to aspartic acid (S1…

   Promoter

   CMV

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dynamin I-K44A-non-tagged

    Plasmid

    #32695

    Depositor

    Robert Lefkowitz

    Article

    Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

    Insert

    Dynamin I (Dnm1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pUAST-SIK3 T196A

    Plasmid

    #52974

    Purpose

    insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

    Depositor

    Biao Wang, Marc Montminy

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    T196A LKB1 phosphorylation defective mutant

    Promoter

    Hsp70

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pRevTRE MKL1-N100 S449A/T450A/S454A

    Plasmid

    #19849

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

    Insert

    MKL1-N100 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    deleted amino acids 1-100 and changed Serine 449,…

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-β-arrestin2 RRK/Q

    Plasmid

    #38261

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Arg233, Arg237, and Lys251 converted to Glutamines

    Promoter

    CMV

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-4T1-Claspin-MΔN

    Plasmid

    #31624

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 899-1206 only

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pSG5-Myr-FLAG-p110α-DM

    Plasmid

    #55713

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    T207D, K227A

    Promoter

    T7

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCIneoLuc-PP1A

    Plasmid

    #128348

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    PP1A (PPP1CA Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX Supt6h-SH2

    Plasmid

    #46518

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Supt6h (SUPT6H Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1317-1441

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GB1-PRD(800-868)-strep

    Plasmid

    #180023

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shNg Lenti FHRC3J1UGW

    Plasmid

    #92233

    Purpose

    lentiviral expression of EGFP and Ng shRNA

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    shRNA targeting Ng
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CMV-cGASΔN-HA (murine)

    Plasmid

    #130915

    Purpose

    Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN murine
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    murine cGAS catalytic domain (aa148-507)

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX Nck-SH2

    Plasmid

    #46457

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nck (NCK1 Human)
    

    Use

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 275-377

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX ShcA-SH2

    Plasmid

    #46486

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcA (SHC1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 476-584

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCherry-C1 SNX9 KR mutant K267N R327N

    Plasmid

    #161994

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K267N R327N

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMA3379

    Plasmid

    #46874

    Purpose

    A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    TRE-Tight

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

    Plasmid

    #161993

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R286A Y287A K288A

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T Doc2beta C2AB(125) WT

    Plasmid

    #134690

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Use

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pRB113.2

    Plasmid

    #181948

    Purpose

    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA Flag-RARalpha (T181A,S445A,S461A)

    Plasmid

    #35556

    Depositor

    Harish Srinivas, Jonathan Kurie

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    T181A, S445A, S461A

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only