We narrowed to 2,433 results for: MEA;
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Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII-ShadowG
Plasmid#167475PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-Spy
Plasmid#90102PurposeIPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.DepositorInsertYcgR (ycgR )
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
SDHC-pHLuorin-sems
Plasmid#107767Purposelabeling of mitochondrial succinate dehydrogenase subunit C, pH measurementsDepositorInsertSubunit C of SDH fused to pHluorin (SDHC Human)
UseTagspHluorinExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-NLucYAP15
Plasmid#107610Purposemeasure LATS activity by Luciferase AssayDepositorInsertNLucYAP15
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector
Plasmid#99292PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FL tension sensor module
Plasmid#101170PurposeExpresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.DepositorInsertYPet(short)-FL-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-Halo-CAMSAP3
Plasmid#191329PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertHalo-CAMSAP3 (Camsap3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-RexYFP
Plasmid#141070PurposeBacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratioDepositorInsertRexYFP
UseTags6xHisExpressionBacterialMutationchanged Leucine 169 to Proline, changed Tyrosine …PromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-12aa-mCit
Plasmid#53237PurposeFluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags12aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO8Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO9Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human-4xUAS
Plasmid#125150PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG
Plasmid#167477PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG
Plasmid#167476PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(T286A)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
Plasmid#117908PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA
Plasmid#118935PurposeUsed to express the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only