We narrowed to 9,824 results for: gne

1. pcDNA3 PM-Ras-LOCKR-S

   Plasmid

   #216498

   Purpose

   FRET-based reporter for Ras activity, plasma membrane localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   PM-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyLigase

   Plasmid

   #51722

   Purpose

   Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

   Depositor

   Mark Howarth

   Article

   Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

   Insert

   SpyLigase
   

   Use

   Tags

   His6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFN18A-HaloTag-SpyCatcher

   Plasmid

   #206041

   Purpose

   HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   HaloTag and SpyCatcher-8His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Myc-GFP-Vpr-RR

   Plasmid

   #83374

   Purpose

   Expression of Myc tagged GFP-Vpr fusion protein

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   Myc-GFP-Vpr
   

   Use

   Tags

   Myc and Myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-51b(+) ChemoG 3.1

   Plasmid

   #193802

   Purpose

   Protein production of ChemoG3.1 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG 3.1
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP: A206K, T225R

   Promoter

   T7

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

   Plasmid

   #40132

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (ELD)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

   Plasmid

   #40131

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (KKR)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSA1_His

   Plasmid

   #202763

   Purpose

   human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eMagAF-EGFP-Mito

   Plasmid

   #162243

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagAF-EGFP-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HSA2_His

    Plasmid

    #202764

    Purpose

    human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TOPO GFP

    Plasmid

    #68488

    Purpose

    TOPO construct expressing GFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Green Fluorescent Protein
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-VSV-G-Myc

    Plasmid

    #80054

    Purpose

    Expression of a Myc tagged VSV-G protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VSV-G-Myc
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. rArc

    Plasmid

    #178206

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rArc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-VSV-G(P127D)-Myc

    Plasmid

    #80055

    Purpose

    Expression of a Myc tagged VSV-G protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VSV-G-Myc(P127D)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P127D

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 Golgi-Ras-LOCKR-S

    Plasmid

    #216499

    Purpose

    FRET-based reporter for Ras activity, Golgi localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Golgi-Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 ER-Ras-LOCKR-S

    Plasmid

    #216500

    Purpose

    FRET-based reporter for Ras activity, ER localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    ER-Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 Mito-Ras-LOCKR-S

    Plasmid

    #216501

    Purpose

    FRET-based reporter for Ras activity, mitochondria localized

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Mito-Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. miRFP718nano-based inflammation reporter

    Plasmid

    #195753

    Purpose

    Reporter of inflamation

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    NF-kB-RE-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM310-Myc

    Plasmid

    #80053

    Purpose

    Expression of a Myc tagged version of the BlaM-Vpr fusion protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-BlaM-Vpr
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal Myc tag added to construct pMM310

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ301

    Plasmid

    #102690

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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