We narrowed to 7,508 results for: ef1a

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pLX-EF1a_GFP

Plasmid

#221481

Purpose

GFP lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

Plasmid

#194464

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z18-PDGFR_EF1a_eGFP

Plasmid

#186304

Purpose

Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z18-PDGFR
eGFP

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_Z17-PDGFR_EF1a_mCherry

Plasmid

#186303

Purpose

Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

Z17-PDGFR
mCherry

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP4-PDGFR_EF1a_eBFP2

Plasmid

#186306

Purpose

Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP4-PDGFR
eBFP2

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg88-PDGFR_EF1a_eBFP2

Plasmid

#186312

Purpose

Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg88-PDGFR
eBFP2

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P9-PDGFR_EF1a_mPlum

Plasmid

#186307

Purpose

Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P9-PDGFR
mPlum

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP10-PDGFR_EF1a_mOrange

Plasmid

#186308

Purpose

Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP10-PDGFR
mOrange

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P3-PDGFR_EF1a_iRFP670

Plasmid

#186305

Purpose

Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P3-PDGFR
iRFP670

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1adCas9VP64T2ABsd-W

Plasmid

#112919

Purpose

Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9VP64-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag-EIF4E

Plasmid

#212657

Purpose

piggyBac vector to express an MLV Gag-EIF4E fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

EIF4E

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD WT TET1

Plasmid

#124082

Purpose

Expression of wild-type catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 catalytic domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.EF1a.ChR2-YFP.WPRE.hGH

Plasmid

#100056

Purpose

AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Insert

ChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162228

Purpose

Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG16LaG2 and IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag-EIF4E-TM

Plasmid

#212658

Purpose

piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

EIF4E (W56L, W102L, W166L)

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDV05.1_pEF1a-iRFP720_frameshifted

Plasmid

#200408

Purpose

Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (frameshift mutant)

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_mCherry

Plasmid

#221482

Purpose

mCherry lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

mCherry

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Ef1a-->flagLaM4_Notch_tTA

Plasmid

#162237

Purpose

Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaM4 mCherry nanobody Notch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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p274 EF1a_EGFP_hH11

Plasmid

#164851

Purpose

EGFP-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

EGFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9

Plasmid

#171691

Purpose

Expresses nSpCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

EF1a

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA Donor_hEF1a

Plasmid

#229779

Purpose

Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1aGFP2AKAT7-W

Plasmid

#159292

Purpose

Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

KAT7 (KAT7 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

CRISPR sites mutated (gRNA ID. 5 and A10)

Promoter

human EF1a promoter

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GFPlig

Plasmid

#162223

Purpose

Lentiviral vector for constitutive expression of GFP ligand in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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RABGEF1A-c014

Plasmid

#175478

Purpose

Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.

Depositor

Opher Gileadi

Insert

RABGEF1 (RABGEF1 Human)

Tags

His6-Zbasic-TEV

Expression

Insect

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_R248W+P278A

Plasmid

#183115

Purpose

Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine and arginine 248 t…

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaCTD_P2A_Hygro_Barcode

Plasmid

#120511

Purpose

Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltaCTD (MYC Human)

Use

Lentiviral

Mutation

Deletion of carboxy-terminal domain

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaNTD_P2A_Hygro_Barcode

Plasmid

#120510

Purpose

Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltaNTD (MYC Human)

Use

Lentiviral

Mutation

Deletion of amino-terminal domain

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaMBI_P2A_Hygro_Barcode

Plasmid

#120504

Purpose

Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltaMBI (MYC Human)

Use

Lentiviral

Mutation

Deletion of MYC Box I domain

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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