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Addgene
Showing: 281 - 320 of 21949 results

  1. pGL2-hOct4 DE-SV40-Luc

    Plasmid
    #26279
    Purpose
    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.
    Depositor
    Rudolf Jaenisch
    Article
    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1
    Insert
    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments below
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26279-plasmid-map-sequence-id-244682

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  12. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 2 (positions 437-527)

    Pooled Library
    #174296
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 1 (positions 333-436)

    Pooled Library
    #174295
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 2 (positions 437-527)

    Pooled Library
    #174294
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 1 (positions 333-436)

    Pooled Library
    #174293
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pCIP

    Plasmid
    #79009
    Purpose
    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.
    Depositor
    Reuben Shaw
    Article
    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCAGGs
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    79009-plasmid-map-sequence-id-151714

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  19. Hamdoun lab FP fusion plasmids

    Plasmid Kit
    #1000000033
    Purpose
    Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)
    Depositor
    Amro Hamdoun
    Available since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).
    Depositor
    Volker Sieber
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  22. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    The modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  23. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. cpFRET

    Plasmid Kit
    #1000000021
    Purpose
    circular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurements
    Depositor
    Olivier Pertz
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals
    Depositor
    Pierre Neveu
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

    Plasmid Kit
    #1000000154
    Purpose
    Vectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.
    Depositor
    Jeffrey Essner
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  38. pBad-mAmetrine

    Plasmid
    #18083
    Purpose
    Bacterial expression of mAmetrine fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May . 5(5):401-3.
    Insert
    mAmetrine fluorescent proteins
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid
    #114110
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.
    Inserts
    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    114110-plasmid-map-sequence-id-252520

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Showing: 281 - 320 of 21949 results