We narrowed to 19,382 results for: INO

1. pAAV-nEF-Con/Fon-iC++-EYFP (AAV8)

   Viral Prep

   #137155-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-iC++-EYFP (#137155). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-iC++-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre and Flp-dependent)

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

   Viral Prep

   #137141-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Flp-dependent)

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-nEF-Coff/Fon-ChR2-mCherry (AAV8)

   Viral Prep

   #137144-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2-mCherry (#137144). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Flp-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   mCherry (Flp-dependent)

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-Ef1a-Con/Foff 2.0-BFP (AAV8)

   Viral Prep

   #137130-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-BFP (#137130). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-BFP plasmid DNA. EF1a-driven, Cre-dependent expression of BFP (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   BFP (Cre-dependent)

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-Ef1a-DIO-PPO-Venus (AAV8)

   Viral Prep

   #139505-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Promoter

   EF1a

   Tags

   Venus (Cre-dependent)

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

   Viral Prep

   #137134-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   mCherry (Flp-dependent)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)

   Viral Prep

   #137127-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   None

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-Ef1a-Con/Foff 2.0-GCaMP6F (AAV8)

   Viral Prep

   #137123-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-GCaMP6F (#137123). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-GCaMP6F plasmid DNA. EF1a-driven, Cre-dependent expression of GCaMP6f (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   None

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-Ef1a-Con/Foff 2.0-mCherry (AAV8)

   Viral Prep

   #137133-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-mCherry (#137133). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-mCherry plasmid DNA. EF1a-driven, Cre-dependent expression of mCherry (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   mCherry (Cre-dependent)

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (AAV8)

    Viral Prep

    #137154-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (#137154). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of NpHR3.3-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Flp-dependent)

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-nEF-Con/Fon-NpHR3.3-EYFP (AAV8)

    Viral Prep

    #137152-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-NpHR3.3-EYFP (#137152). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of NpHR3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre and Flp-dependent)

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-nEF-Con/Fon-ChR2-mCherry (AAV8)

    Viral Prep

    #137142-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2-mCherry (#137142). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Cre and Flp-dependent expression of channelrhodopsin fused to mCherry for optogenetic activation.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    mCherry (Cre and Flp-dependent)

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-FLEX-EGFPL10a (AAV Retrograde)

    Viral Prep

    #98747-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Nathaniel Heintz, Alexander Nectow, Eric Schmidt

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Promoter

    EF1a

    Tags

    EGFP (Cre-dependent)

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Ef1a-Coff/Fon-GCaMP6F (AAV8)

    Viral Prep

    #137124-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6F (#137124). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6F plasmid DNA. EF1a-driven, Flp-dependent expression of GCaMP6f (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    None

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-Ef1a-DIO-PPO-Venus (AAV9)

    Viral Prep

    #139505-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    EF1a

    Tags

    Venus (Cre-dependent)

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Ef1a-Coff/Fon-BFP (AAV8)

    Viral Prep

    #137131-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-BFP (#137131). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-BFP plasmid DNA. EF1a-driven, Flp-dependent expression of BFP (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    BFP (Flp-dependent)

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-nEF-Coff/Fon-iC++-EYFP (AAV8)

    Viral Prep

    #137157-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-iC++-EYFP (#137157). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-iC++-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Flp-dependent)

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

18. AAV pmSyn1-EBFP-Cre (AAV5)

    Viral Prep

    #51507-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV pmSyn1-EBFP-Cre (#51507). In addition to the viral particles, you will also receive purified AAV pmSyn1-EBFP-Cre plasmid DNA. EBFP-Cre expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Promoter

    pmSyn1

    Tags

    EBFP

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-Ef1a-Con/Fon-BFP (AAV8)

    Viral Prep

    #137129-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-BFP (#137129). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-BFP plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of BFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    BFP (Cre and Flp-dependent)

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-Ef1a-Con/Foff 2.0-oScarlet (AAV8)

    Viral Prep

    #137137-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-oScarlet (#137137). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-oScarlet plasmid DNA. EF1a-driven, Cre-dependent expression of oScarlet (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    oScarlet (Cre-dependent)

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

21. pAAV-nEF-Con/Foff 2.0-iC++-EYFP (AAV8)

    Viral Prep

    #137156-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-iC++-EYFP (#137156). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-iC++-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of chloride-conducting channelrhodopsin iC++-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre-dependent)

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

22. pAAV-CAG-DIO-PPO-Venus (AAV5)

    Viral Prep

    #139504-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-DIO-PPO-Venus (#139504). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-PPO-Venus plasmid DNA. CAG-driven, Cre-dependent expression of Venus-tagged parapinopsin. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Promoter

    CAG

    Tags

    Venus

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

23. AAV phSyn1(S)-DreO-bGHpA (AAV5)

    Viral Prep

    #50363-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV phSyn1(S)-DreO-bGHpA (#50363). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-DreO-bGHpA plasmid DNA. hSyn-driven Dre recombinase expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Promoter

    Syn

    Tags

    None

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

24. pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral Prep

    #137139-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP (#137139). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of ChR2(E123T/T159C)-EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre and Flp-dependent)

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

25. AAV phSyn1(S)-DreO-bGHpA (AAV Retrograde)

    Viral Prep

    #50363-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV phSyn1(S)-DreO-bGHpA (#50363). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-DreO-bGHpA plasmid DNA. hSyn-driven Dre recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Promoter

    Syn

    Tags

    None

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

26. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (AAV1)

    Viral Prep

    #65453-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (#65453). In addition to the viral particles, you will also receive purified AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA plasmid DNA. TRE-driven, Cre and Flp-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Promoter

    tetO

    Tags

    EGFP (Cre and Flp-dependent)

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

27. pAAV-nEF-Con/Fon/Von eYFP (AAV8)

    Viral Prep

    #137164-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon/Von eYFP (#137164). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon/Von eYFP plasmid DNA. nEF-driven, Cre, Flp and VCre-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre, Flp and VCre-dependent)

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

28. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBGE1.0

    Plasmid

    #165986

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAGE1.0

    Plasmid

    #165983

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. INCTbiosyn-pUB-HspINT7

    Plasmid

    #127515

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 7.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 7 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    In 2126 position an G mutated for an T changed th…

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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34. pscALPSpuro-RsACE2

    Plasmid

    #159665

    Purpose

    Expresses Rhinolophus sinicus ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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35. A1-LCD Aro+

    Plasmid

    #169717

    Purpose

    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.

    Depositor

    Tanja Mittag

    Article

    Martin et al Science. 2020 Feb 7;367(6478):694-699. d

    Insert

    hnRNPA1 (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hnRNPA1-A Low complexity domain including amino a…

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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36. pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP (AAV8)

    Viral Prep

    #137149-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP (#137149). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of Arch3.3-EYFP (inhibited in presence of Flp) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre-dependent)

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

37. AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)

    Viral Prep

    #51669-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Promoter

    Syn

    Tags

    None

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

38. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet (AAV8)

    Viral Prep

    #137161-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet (#137161). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    oScarlet (Cre-dependent)

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

39. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)

    Viral Prep

    #137163-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre-dependent)

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

40. pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP (AAV8)

    Viral Prep

    #137140-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP (#137140). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Flp) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Cre-dependent)

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only