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Showing: 281 - 320 of 8720 results

  2. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

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  3. pET15-EF1p3_mFAP2b

    Plasmid
    #159470
    Purpose
    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p3_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159470-plasmid-map-sequence-id-316659

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  8. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid
    #200408
    Purpose
    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    iRFP720 (frameshift mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200408-plasmid-map-sequence-id-396809

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  9. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

    Plasmid
    #162804
    Purpose
    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080
    Insert
    Ub-BsmBI_IRES_Blasticidin
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    162804-plasmid-map-sequence-id-318670

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  10. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  11. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

    Plasmid
    #194461
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194461-plasmid-map-sequence-id-384277

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  12. PB_TRE_AP4-PDGFR_EF1a_eBFP2

    Plasmid
    #186306
    Purpose
    Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    AP4-PDGFR
    eBFP2
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186306-plasmid-map-sequence-id-366944

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  20. pKDR-EF1a.4

    Plasmid
    #183571
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183571-plasmid-map-sequence-id-365376

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  21. pKDR-EF1a.2

    Plasmid
    #183569
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183569-plasmid-map-sequence-id-365375

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  22. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

    Plasmid
    #194463
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194463-plasmid-map-sequence-id-384263

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  23. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

    Plasmid
    #120514
    Purpose
    Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC Ser62Ala (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Point mutation changing Serine to Alanine at amin…
    Promoter
    EF1a
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120514-plasmid-map-sequence-id-234976

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  35. PB_TRE_sg83-PDGFR_EF1a_iRFP670

    Plasmid
    #186311
    Purpose
    Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg83-PDGFR
    iRFP670
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186311-plasmid-map-sequence-id-366938

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  36. PB_TRE_sg88-PDGFR_EF1a_eBFP2

    Plasmid
    #186312
    Purpose
    Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg88-PDGFR
    eBFP2
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186312-plasmid-map-sequence-id-366935

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  37. PB_TRE_P9-PDGFR_EF1a_mPlum

    Plasmid
    #186307
    Purpose
    Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    P9-PDGFR
    mPlum
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186307-plasmid-map-sequence-id-366942

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  38. PB_TRE_AP10-PDGFR_EF1a_mOrange

    Plasmid
    #186308
    Purpose
    Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    AP10-PDGFR
    mOrange
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186308-plasmid-map-sequence-id-366950

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  39. PB_TRE_P3-PDGFR_EF1a_iRFP670

    Plasmid
    #186305
    Purpose
    Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    P3-PDGFR
    iRFP670
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186305-plasmid-map-sequence-id-366925

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Showing: 281 - 320 of 8720 results