We narrowed to 11,201 results for: NSI;

1. pAM4731

   Plasmid

   #85127

   Purpose

   NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

   Depositor

   Susan Golden

   Article

   Chang et al Science. 2015 Jul 17;349(6245):324-8. do

   Insert

   PkaiBC-KaiB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-2T-TRR-C421-G2304D

   Plasmid

   #182845

   Purpose

   mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   mutation G2304D (GGC/GAC)

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human CRISPR Poison Exon Knockout Library

   Pooled Library

   #138084

   Purpose

   The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).

   Depositor

   Robert Bradley

   Article

   Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pGM202T7

   Plasmid

   #69993

   Purpose

   E. coli/Streptomyces expression vector containing the T7-promoter

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aphII
   tsr
   rep
   sso
   T7 promoter
   dso
   His-Orf
   

   Use

   Tags

   N-terminal and C-terminal His tag encoding sequen…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet rtTA G72V SE Δsplice GP Luc

   Plasmid

   #194328

   Purpose

   Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

   Depositor

   Alexander McLellan

   Article

   Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Tet-responsive promotor related mutations: G72V i…

   Promoter

   Tight tet-responsive promotor

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ma-PylRS-lib1

   Pooled Library

   #224585

   Purpose

   Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Expression

   Bacterial

   Species

   Methanomethylophilus alvus

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

7. pLCKO2::TKOv3

   Pooled Library

   #125517

   Purpose

   Human CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backbone

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Mix.2-lux

   Plasmid

   #20893

   Depositor

   Stefano Piccolo

   Article

   Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

   Insert

   -290 Mix.2 promoter luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Barcode Library 0 (BCL0)

   Pooled Library

   #179851

   Purpose

   Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

   Depositor

   Jason Moffat

   Article

   Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

10. Barcode Library 1 (BCL1)

    Pooled Library

    #179852

    Purpose

    Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

    Depositor

    Jason Moffat

    Article

    Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

    Expression

    Mammalian

    Use

    Lentiviral

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

11. MscL(G22S)61-cpGFP-ERexp

    Plasmid

    #195311

    Purpose

    Fluorescent reporter/biosensor for membrane tension

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)61-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MscL(G22S)-c-term-cpGFP-ERexp

    Plasmid

    #195346

    Purpose

    Control plasmid for the MscL tension biosensor

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)-c-term-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd2

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Actinin-sstFRET-GR

    Plasmid

    #83416

    Purpose

    FRET-based actinin tension sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    Actinin-sstFRET-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

15. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pAC-EsaR

    Plasmid

    #47643

    Purpose

    wt esaR gene under control of Plac in pACYC184

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    Plac-esaR-terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAC-EsaR-D91G

    Plasmid

    #47646

    Purpose

    pAC-EsaR with a a272g mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCS-PluxIlux

    Plasmid

    #47641

    Purpose

    wt luxI promoter in pCS26

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    PluxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PluxI

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pETSXM2-pLacI-mtrC

    Plasmid

    #174615

    Purpose

    Bacterial expression of mtrC from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    MtrC
    

    Use

    Synthetic Biology; Shuttle vector in e. coli and …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-EsaR-V220A

    Plasmid

    #47645

    Purpose

    pAC-EsaR with a c659t mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-V220A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Valine 220 to Alanine

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only