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We narrowed to 198 results for: araf

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Showing: 141 - 160 of 198 results

  3. pUC19-π-EB1_EF1α-mCherry

    Plasmid
    #197429
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197429-plasmid-map-sequence-id-388078

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  6. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  7. pEB1N-LZ-mCherry-LOV2(wt)

    Plasmid
    #107693
    Purpose
    N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends
    Depositor
    Torsten Wittmann
    Article
    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038
    Insert
    MAPRE1 (MAPRE1 Human)
    Tags
    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…
    Expression
    Mammalian
    Mutation
    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107693-plasmid-map-sequence-id-209378

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  10. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid
    #125712
    Purpose
    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    eOPN3
    Use
    AAV
    Tags
    Rho1D4 and mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 331-429
    Promoter
    short version of αCamKinase II promoter (0.4kb)
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    125712-plasmid-map-sequence-id-247206

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  14. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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Showing: 141 - 160 of 198 results