Skip to main content
Addgene
Showing: 141 - 160 of 3138 results

  2. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

    Enlarge

  3. PML-3/pSG5

    Plasmid
    #50939
    Purpose
    expression of PML with Sumo sites destroyed
    Depositor
    Pier Pandolfi
    Article
    Zhong et al Blood. 2000 May 1;95(9):2748-52.
    Insert
    PML-3 (PML Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K65R, K160R, K490R (Sumo modification sites)
    Promoter
    SV40
    Available since
    Jan. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. SOX2-3'gRNA

    Plasmid
    #215543
    Purpose
    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in
    Depositor
    Kai Wang
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215543-plasmid-map-sequence-id-424976

    Enlarge

Showing: 141 - 160 of 3138 results