We narrowed to 281 results for: dcas9 vp64

1. pBUN6A11

   Plasmid

   #50579

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   dCas9-VP64
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG, HA, and NLS

   Expression

   Mutation

   dCas9 = defective in DNA cleavage; 4×minimal VP16…

   Promoter

   OsU3p and Ubi1p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ173

   Plasmid

   #99907

   Purpose

   Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Insert

   pco-dCas9-VP64-T2A-MS2-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJZC638

   Plasmid

   #62283

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Nuclease activity has been deactivated by mutatio…

   Promoter

   pAdh and pGal10

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   VP64 activator fusion; NLS fusion

   Promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJZC519

   Plasmid

   #62278

   Purpose

   Yeast dCas9-VP64 expression plasmid

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   dCas9-VP64
   

   Use

   Tags

   Has a VP64 fusion

   Expression

   Yeast

   Mutation

   Has mutations in the RuvC1 and HNH domains to ren…

   Promoter

   pTdh3

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJZC595

   Plasmid

   #62322

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Has nuclease-inactivating mutations in the RuvC1 …

   Promoter

   Tdh3 and pAdh

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-VdC9BV

   Plasmid

   #62195

   Purpose

   Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

   Depositor

   Kam Leong

   Article

   Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

   Insert

   VP64dCas9BFPVP64
   

   Use

   Lentiviral

   Tags

   Two VP64s tagged to dCas9 fused to BFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive) Cas9 (dCas9)

   Promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-scFv-p65-HSF1-Blast

   Plasmid

   #192652

   Purpose

   3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-p65-HSF1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-U6-gRNA-TRE3G-TdTomato

   Plasmid

   #192658

   Purpose

   A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, TRE3G

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR-U6-gRNA-TRE3G-Nluc

    Plasmid

    #192659

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR-U6-gRNA-miniCMV-Nluc

    Plasmid

    #192657

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-dSa-VPR

    Plasmid

    #99651

    Purpose

    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

    Depositor

    George Church

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65-RTA activator

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. OA-986D

    Plasmid

    #125001

    Purpose

    Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. OA-986E

    Plasmid

    #125002

    Purpose

    Expresses dCas9-VP64 driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bottleneck

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiU6-hMYOD1 gRNA_1-MS2-Puro

    Plasmid

    #192673

    Purpose

    Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNA #1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

    Plasmid

    #192676

    Purpose

    Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. M_ST1n_VPR

    Plasmid

    #63799

    Purpose

    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    ST1-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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