We narrowed to 338 results for: idua
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Plasmid#169734PurposeEqualizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInserttetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_CV2
Plasmid#167165PurposepgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
mKO-Alpha-Actinin-19
Plasmid#57831DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi1-LgB91
Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorInsertGαi1-LgB91 (GNAI1 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-ch-TOG-His6
Plasmid#69108PurposeFor bacterial expression of recombinant human ch-TOG tagged with MBP and His.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorInsertGαq-LgB97 (GNAQ Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
Plasmid#31483DepositorInsertTraffic Light Reporter 2.1 (V2468 zfn target) EF Puro
UseLentiviralTagsExpressionMutationTraffic Light 2.0 series have a +3 PEST sequence …PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette
Plasmid#169737PurposeEqualizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2–mPKCε-CAT-HA
Plasmid#190483PurposeExpresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAXDepositorInsertmCherry-CRY2–mPKCε-CAT-HA (Prkce Synthetic, Mouse)
UseTagsFused to CRY2, HA, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1(long)-His6
Plasmid#69110PurposeFor bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.DepositorInsertG2- and S-phase Expressed 1 (GTSE1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
P63-sp64 Poly A
Plasmid#59435Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 63
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P29-sp64 Poly A
Plasmid#59434Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 29
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
P21-sp64 Poly A
Plasmid#59433Purposesynthetic poly A transcripts of VZVDepositorInsertVZV ORF 21
UseMakes synthetic transcripts that contains poly aTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid with onboard mCherry cassette
Plasmid#169735PurposeEqualizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pABpuro-BluF
Plasmid#46824Purposeluciferase reporter for BmalI promoterDepositorInsertBmal1 (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationPromoterBmal1Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ash-mCerulean
Plasmid#69579PurposeExpresses p53 tagged with mCeruleanDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorInsertGαi3-LgB91 (GNAI3 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.D2-NP
Plasmid#134366PurposeNanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.DepositorInsertD2-NP (DRD2 Human)
UseTagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorInsertGαi2-LgB91 (GNAI2 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorInsertGα12-LgB115 (GNA12 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only